Flavonoids induce temporal shifts in gene-expression of nod-box controlled loci in Rhizobium sp NGR234

被引:95
作者
Kobayashi, H
Naciri-Graven, Y
Broughton, WJ
Perret, X
机构
[1] Univ Geneva, LBMPS, CH-1292 Geneva, Switzerland
[2] Conservatoire & Jardins Bot, Lab Genet & Phylogenie Mol, CH-1292 Geneva, Switzerland
关键词
D O I
10.1046/j.1365-2958.2003.03841.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rhizobia, soil bacteria of the Rhizobiales, enter the roots of homologous legumes, where they induce the formation of nitrogen-fixing nodules. Signals emanating from both symbiotic partners control nodule development. Efficient nodulation requires precise, temporal regulation of symbiotic genes. Roots continuously release flavonoids that interact with transcriptional activators of the LysR family. NodD proteins, which are members of this family, act both as sensors of the environment and modulate the expression of genes preceded by conserved promoter sequences called nod-boxes. The symbiotic plasmid of the broad host-range Rhizobium sp. NGR234 caries 19 nod-boxes (NB1 to NB19), all of which were cloned upstream of a lacZ-reporter gene. A flavonoid, daidzein was able to induce 18 of the 19 nod-boxes in a NodD1-dependent manner. Interestingly, induction of four nod-boxes (NB6, NB15, NB16 and NB17) is highly dependent on NodD2 and was delayed in comparison with the others. In turn, NodD2 is involved in the repression of the NB8 nodABCIJnolOnoeI operon. Activation of transcription of nodD2 is also dependent on flavonoids despite the absence of a nod-box like sequence in the upstream promoter region. Mutational analysis showed that syrM 2 (another member of the LysR family), which is controlled by NB19, is also necessary for expression of nodD 2. Thus, NodD1, NodD2 and SyrM2 co-modulate a flavonoid-inducible regulatory cascade that coordinates the expression of symbiotic genes with nodule development.
引用
收藏
页码:335 / 347
页数:13
相关论文
共 81 条
[1]   Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid [J].
Barnett, MJ ;
Fisher, RF ;
Jones, T ;
Komp, C ;
Abola, AP ;
Barloy-Hubler, F ;
Bowser, L ;
Capela, D ;
Galibert, F ;
Gouzy, J ;
Gurjal, M ;
Hong, A ;
Huizar, L ;
Hyman, RW ;
Kahn, D ;
Kahn, ML ;
Kalman, S ;
Keating, DH ;
Palm, C ;
Peck, MC ;
Surzycki, R ;
Wells, DH ;
Yeh, KC ;
Davis, RW ;
Federspiel, NA ;
Long, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9883-9888
[2]  
Barnett MJ, 1998, GENETICS, V148, P19
[3]   Transcription start sites for syrM and nodD3 flank an insertion sequence relic in Rhizobium meliloti [J].
Barnett, MJ ;
Rushing, BG ;
Fisher, RF ;
Long, SR .
JOURNAL OF BACTERIOLOGY, 1996, 178 (07) :1782-1787
[4]   NolL of Rhizobium sp strain NGR234 is required for O-acetyltransferase activity [J].
Berck, S ;
Perret, X ;
Quesada-Vincens, D ;
Promé, JC ;
Broughton, WJ ;
Jabbouri, S .
JOURNAL OF BACTERIOLOGY, 1999, 181 (03) :957-964
[5]  
BERINGER JE, 1974, J GEN MICROBIOL, V84, P188
[6]   SEQUENCE OF PSI, A GENE ON THE SYMBIOTIC PLASMID OF RHIZOBIUM-PHASEOLI WHICH INHIBITS EXOPOLYSACCHARIDE SYNTHESIS AND NODULATION AND DEMONSTRATION THAT ITS TRANSCRIPTION IS INHIBITED BY PSR, ANOTHER GENE ON THE SYMBIOTIC PLASMID [J].
BORTHAKUR, D ;
JOHNSTON, AWB .
MOLECULAR & GENERAL GENETICS, 1987, 207 (01) :149-154
[7]   Keys to symbiotic harmony [J].
Broughton, WJ ;
Jabbouri, S ;
Perret, X .
JOURNAL OF BACTERIOLOGY, 2000, 182 (20) :5641-5652
[8]   IDENTIFICATION OF RHIZOBIUM PLASMID SEQUENCES INVOLVED IN RECOGNITION OF PSOPHOCARPUS, VIGNA, AND OTHER LEGUMES [J].
BROUGHTON, WJ ;
WONG, CH ;
LEWIN, A ;
SAMREY, U ;
MYINT, H ;
MEYER, H ;
DOWLING, DN ;
SIMON, R .
JOURNAL OF CELL BIOLOGY, 1986, 102 (04) :1173-1182
[9]   PLASMID-LINKED NIF AND NOD GENES IN FAST-GROWING RHIZOBIA THAT NODULATE GLYCINE MAX, PSOPHOCARPUS-TETRAGONOLOBUS, AND VIGNA-UNGUICULATA [J].
BROUGHTON, WJ ;
HEYCKE, N ;
MEYER, H ;
PANKHURST, CE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (10) :3093-3097
[10]   NOLR CONTROLS EXPRESSION OF THE RHIZOBIUM-MELILOTI NODULATION GENES INVOLVED IN THE CORE NOD FACTOR SYNTHESIS [J].
CREN, M ;
KONDOROSI, A ;
KONDOROSI, E .
MOLECULAR MICROBIOLOGY, 1995, 15 (04) :733-747