Isolation and evaluation of antioxidants from leaves of a Tunisian cultivar olive tree

被引:157
作者
Bouaziz, M [1 ]
Sayadi, S [1 ]
机构
[1] Ctr Biotechnol Sfax, Lab Bioproc, Sfax, Tunisia
关键词
chemlali olive leaf; oleuropein; hydroxytyrosol; flavonoids; acid hydrolysis; antioxidant;
D O I
10.1002/ejlt.200501166
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Quantitative and qualitative determinations of phenolic compounds were carried out on Chemlali olive leaves, a by-product of olive tree pruning. The evolution of the amount of the major phenolic compound was monitored from July 2003 to March 2004. Quantitative analysis was performed by high-performance liquid chromatography (HPLC), which revealed that oleuropein was always the major compound. Its concentration reached 6.8 g per 100 g of fresh olive leaves, while monomeric phenols were the minor compounds. Beside oleuropein, six flavonoids (luteolin 7-O-glucoside, luteolin 7-O-rutinoside, apigenin 7-O-glucoside, rutin, luteolin and apigenin) were identified. The identification was based on separation by HPLC equipped with a diode array detector, followed by liquid chromatography-mass spectrometry analysis. A relative high amount of purified hydroxytyrosol (2.3g per 100g of fresh olive leaves) was obtained in short time by a simple hydrolysis reaction of Olea europaea leaf extract and by purification using a C-18 silica gel column. The antioxidant activities of the extracts and the purified compounds were evaluated by measuring the radical-scavenging effect on 1,1-diphenyl-2-picrylhydrazyl and by using the P-carotene linoleate model assay. Hydroxytyrosol and hydrolysate products have the highest antioxiclant activities.
引用
收藏
页码:497 / 504
页数:8
相关论文
共 35 条
[1]   Toward a high yield recovery of antioxidants and purified hydroxytyrosol from olive mill wastewaters [J].
Allouche, N ;
Fki, I ;
Sayadi, S .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (02) :267-273
[2]  
Allouche N., 2004, J SOC CHIMIQUE TUNIS, V6, P33
[3]   Phenolic profile in the quality control of walnut (Juglans regia L.) leaves [J].
Amaral, JS ;
Seabra, RM ;
Andrade, PB ;
Valentao, P ;
Pereira, JA ;
Ferreres, F .
FOOD CHEMISTRY, 2004, 88 (03) :373-379
[4]  
[Anonymous], 1985, CHIRON, V15, P91
[5]   Antioxidant activity of phenolics extracted from Olea europaea L-leaves [J].
Benavente-García, O ;
Castillo, J ;
Lorente, J ;
Ortuño, A ;
Del Rio, JA .
FOOD CHEMISTRY, 2000, 68 (04) :457-462
[6]   Identification and antioxidant potential of flavonoids and low molecular weight phenols in olive cultivar Chemlali growing in Tunisia [J].
Bouaziz, M ;
Grayer, RJ ;
Simmonds, MSJ ;
Damak, M ;
Sayadi, S .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (02) :236-241
[7]   Comparative study on phenolic content and antioxidant activity during maturation of the olive cultivar Chemlali from Tunisia [J].
Bouaziz, M ;
Chamkha, M ;
Sayadi, S .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (17) :5476-5481
[8]   Olea europaea L. leaf extract and derivatives:: Antioxidant properties [J].
Briante, R ;
Patumi, M ;
Terenziani, S ;
Bismuto, E ;
Febbraio, F ;
Nucci, R .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (17) :4934-4940
[9]   Production of highly purified hydroxytyrosol from Olea europaea leaf extract biotransformed by hyperthermophilic β-glycosidase [J].
Briante, R ;
Patumi, M ;
Febbraio, F ;
Nucci, R .
JOURNAL OF BIOTECHNOLOGY, 2004, 111 (01) :67-77
[10]   Bioactive derivatives from oleuropein by a biotransformation on Olea europaea leaf extracts [J].
Briante, R ;
La Cara, F ;
Febbraio, F ;
Patumi, M ;
Nucci, R .
JOURNAL OF BIOTECHNOLOGY, 2002, 93 (02) :109-119