New approaches to in vitro models of blood-brain barrier drug transport

被引：108

作者：

Terasaki, T ^{[1
]}

Ohtsuki, S

Hori, S

Takanaga, H

Nakashima, E

Hosoya, K

机构：

[1] Tohoku Univ, Dept Mol Biopharm & Genet, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, New Ind Creat, Aoba Ku, Sendai, Miyagi 9808578, Japan

[3] Kyoritsu Coll Pharmaceut Sci, Dept Pharmaceut, Tokyo 1058512, Japan

[4] Toyama Med & Pharmaceut Univ, Sugitani, Toyama 9300194, Japan

来源：

DRUG DISCOVERY TODAY | 2003年 / 8卷 / 20期

关键词：

D O I：

10.1016/S1359-6446(03)02858-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The pharmaceutical industry has been searching for an in vitro blood-brain barrier (BBB) model that preserves in vivo transporter functions in CNS drug discovery and development. The application of conditionally immortalized cell lines derived from transgenic animals harboring temperature-sensitive SV40 large T-antigen gene, is a rational and promising approach to such a workable in vitro BBB model. The established brain capillary endothelial cell lines retain the in vivo transport rate of several compounds and various forms of gene expression. Furthermore, this new approach has enabled the development of stable and reproducible co-culture models with a pericyte cell line and/or an astrocyte cell line.

引用

页码：944 / 954

页数：11

共 81 条

[21] Localization of the organic anion transporting polypeptide 2 (Oatp2) in capillary endothelium and choroid plexus epithelium of rat brain [J].