Determination of trimethoprim-sulfamethoxazole resistance in Streptococcus pneumoniae by using the E test with Mueller-Hinton agar supplemented with sheep or horse blood may be unreliable

被引:10
作者
Lovgren, M
Dell'Acqua, L
Palacio, R
Echániz-Aviles, G
Soto-Noguerón, A
Castaneda, E
Agudelo, CI
Heitmann, I
Brandileone, MC
Zanella, RC
Rossi, A
Pace, J
Talbot, JA
机构
[1] Natl Ctr Streptococcus, Edmonton, AB, Canada
[2] Minsisterio Salud Publ, Dept Lab Salud, Montevideo, Uruguay
[3] Inst Nacl Salud Publ, Cuernavaca, Morelos, Mexico
[4] Inst Nacl Salud, Bogota, Colombia
[5] Inst Salud Publ, Santiago, Chile
[6] Inst Adolfo Lutz, Sao Paulo, Brazil
[7] Inst Nacl Microbiol Dr Carlos Malbran, Buenos Aires, DF, Argentina
关键词
D O I
10.1128/JCM.37.1.215-217.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An international, multicenter study compared trimethoprim-sulfamethoxazole MICs for 743 Streptococcus pneumoniae isolates (107 to 244 isolates per country) by E test, using Mueller-Hinton agar supplemented with 5% defibrinated horse blood or 5% defibrinated sheep blood, with MICs determined by the National Committee for Clinical Laboratory Standards broth microdilution reference method. Agreement within 1 log(2) dilution and minor error rates were 69.3 and 15.5%, respectively, on sheep blood-supplemented agar and 76.9 and 13.6%, respectively, with horse blood as the supplement. Significant interlaboratory variability was observed. E test may not be a reliable method for determining the resistance of pneumococci to trimethoprim-sulfamethoxazole.
引用
收藏
页码:215 / 217
页数:3
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