Expression of β-synuclein in normal human astrocytes

被引：19

作者：

Tanji, K

Mori, F

Nakajo, S

Imaizumi, T

Yoshida, H

Hirabayashi, T

Yoshimoto, M

Satoh, K

Takahashi, H

Wakabayashi, K

机构：

[1] Hirosaki Univ, Sch Med, Inst Brain Sci, Dept Mol Biol, Hirosaki, Aomori 0368562, Japan

[2] Hirosaki Univ, Sch Med, Inst Brain Sci, Dept Vasc Biol, Hirosaki, Aomori 0368562, Japan

[3] Showa Univ, Sch Pharmaceut Sci, Biol Chem Lab, Tokyo 142, Japan

[4] Taisho Pharmaceut Co Ltd, Mol Biol Lab, Omiya, Saitama, Japan

[5] Niigata Univ, Brain Res Inst, Dept Pathol, Niigata, Japan

来源：

NEUROREPORT | 2001年 / 12卷 / 13期

关键词：

astrocyte; culture; glial cell; human brain; beta-synuclein;

D O I：

10.1097/00001756-200109170-00018

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Although alpha- and beta -synucleins are expressed predominantly in presynaptic nerve terminals, recent studies have demonstrated that alpha -synuclein is also expressed in cultured astrocytes and oligodendrocytes. We determined whether alpha -synuclein might be expressed in astrocytes. alpha -Synuclein mRNA and protein were detected in normal human astrocytes in culture, and immunofluorescent staining showed that beta -synuclein protein was expressed within the cytoplasm and nucleus. Furthermore, beta -synuclein immunoreactivity was present in astrocytes, but not in oligodendrocytes, in normal human brain tissues. Ultrastructurally, beta -synuclein immunoreactivity was found in the cytoplasm of astrocytes, in association with the plasma membrane, ribosomes, rough endoplasmic reticulum and the nuclear outer membrane. The novel expression of beta -synuclein in astrocytes may provide an important insight about the role of this protein. NeuroReport 12:2845-2848 (C) 2001 Lippincott Williams & Wilkins.

引用

页码：2845 / 2848

页数：4

共 25 条

[1] Argyrophilic glial inclusions in the midbrain of patients with Parkinson's disease and diffuse Lewy body disease are immunopositive for NACP/α-synuclein [J].