Chemical and molecular characterization of Phomopsis and Cytospora-like endophytes from different host plants in Brazil

被引：29

作者：

Abreu, Lucas M. ^{[1
,2
]}

Costa, Sarah S. ^{[1
]}

Pfenning, Ludwig H. ^{[1
]}

Takahashi, Jacqueline A. ^{[2
]}

Larsen, Thomas O. ^{[3
]}

Andersen, Birgitte ^{[3
]}

机构：

[1] Univ Fed Lavras, Dept Phytopathol, BR-37200000 Lavras, MG, Brazil

[2] Univ Fed Minas Gerais, Dept Chem, BR-31270901 Belo Horizonte, MG, Brazil

[3] Tech Univ Denmark, Dept Syst Biol, Ctr Microbial Biotechnol, DK-2800 Lyngby, Denmark

来源：

FUNGAL BIOLOGY | 2012年 / 116卷 / 02期

关键词：

Chemotaxonomy; Diaporthales; Mass spectrometry; Natural product chemistry; Phylogeny; LIQUID-CHROMATOGRAPHY; FUNGAL METABOLITES; DIAPORTHE; CHEMOTAXONOMY; MYCOTOXINS; DIVERSITY; TAXONOMY; DATABASE; COMPLEX;

D O I：

10.1016/j.funbio.2011.11.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Phomopsis and related taxa comprise important endophytic and plant pathogenic species, and are known for the production of a diverse array of secondary metabolites. Species concepts within this group based on morphological characters and assumed host specificity do not reflect phylogenetic affinities. Additional phenotypic characters, such as profiles of secondary metabolites, are needed for practical species recognition. We investigated 36 strains of Phomopsis spp. and Cytospora-like fungi, obtained as endophytes of different host plants in Brazil, using metabolite profiling based on HPLC UV/liquid chromatography - mass spectrometry (LC-MS) combined with cluster analysis of the results. Strains were also subjected to phylogenetic analyses based on internal transcribed spacer (ITS) rDNA. Six chemotypes were identified. Chemotypes 1-5 contained Phomopsis strains, while Cytospora-like strains formed the chemotype 6. Strains of chemotype 1 typically produced alternariols, altenusin, altenuene, cytosporones, and dothiorelones. Alternariol and seven unknown compounds were consistently produced by strains of chemotype 2. Members of chemotypes 3-5 produced poor metabolite profiles containing few chemical markers. Cytospora-like endophytes (chemotype 6) produced a characteristic set of metabolites including cytosporones and dothiorelones. Bayesian and Maximum Parsimony (MP) trees classified strains of each chemotype into single phylogenetic lineages or closely related groups. Strains of chemotypes 1 and 2 formed a monophyletic group along with Diaporthe neotheicola. The remaining Phomopsis strains formed monophyletic (chemotype 4) or polyphyletic (chemotypes 3 and 5) lineages inside a large and well supported clade. Cytospora-like strains formed a monophyletic lineage located at an intermediary position between Diaporthe/Phomopsis and Valsa/Cytospora clades. The combined results show that the production of secondary metabolites by Phomopsis and related Diaporthales may be species-specific, giving support to the use of metabolite profiling and chemical classification for phenotypic recognition and delimitation of species. (C) 2011 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：249 / 260

页数：12

共 46 条

[1] Cytosporones O, P and Q from an endophytic Cytospora sp. [J].

Abreu, Lucas M. ;

Phipps, Richard K. ;

Pfenning, Ludwig H. ;

Gotfredsen, Charlotte H. ;

Takahashi, Jacqueline A. ;

Larsen, Thomas O. .

TETRAHEDRON LETTERS, 2010, 51 (13) :1803-1805

[2]

Adams G C., 2005, Studies in Mycology, V52, P1

[3] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[4] CONSISTENT PRODUCTION OF PHENOLIC-COMPOUNDS BY PENICILLIUM-BREVICOMPACTUM FOR CHEMOTAXONOMIC CHARACTERIZATION [J].

ANDERSEN, B .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1991, 60 (02) :115-123

[5] Secondary metabolite profiling of Alternaria dauci, A. porri, A. solani, and A. tomatophila [J].

Andersen, Birgitte ;

Dongo, Anita ;

Pryor, Barry M. .

MYCOLOGICAL RESEARCH, 2008, 112 :241-250

[6] A polyphasic approach to the taxonomy of the Alternaria infectoria species-group [J].

Andersen, Birgitte ;

Sorensen, Jens Laurids ;

Nielsen, Kristian Fog ;

van den Ende, Bert Gerrits ;

de Hoog, Sybren .

FUNGAL GENETICS AND BIOLOGY, 2009, 46 (09) :642-656

[7] Discovery of the parnafungins, antifungal metabolites that inhibit mRNA polyadenylation, from the Fusarium larvarum complex and other Hypocrealean fungi [J].

Bills, Gerald F. ;

Platas, Gonzalo ;

Overy, David P. ;

Collado, Javier ;

Fillola, Asuncion ;

Jimenez, Maria Rosa ;

Martin, Jesus ;

del Val, Antonio Gonzalez ;

Vicente, Francisca ;

Tromo, J. Ruben ;

Pelaez, Fernando ;

Calati, Kathleen ;

Harris, Guy ;

Parish, Craig ;

Xu, Deming ;

Roemer, Terry .

MYCOLOGIA, 2009, 101 (04) :449-472

[8] The cytosporones, new octaketide antibiotics isolated from an endophytic fungus [J].

Brady, SF ;

Wagenaar, MM ;

Singh, MP ;

Janso, JE ;

Clardy, J .

ORGANIC LETTERS, 2000, 2 (25) :4043-4046

[9] Diversity and host preference of leaf endophytic fungi in the Iwokrama Forest Reserve, Guyana [J].

Cannon, PF ;

Simmons, CM .

MYCOLOGIA, 2002, 94 (02) :210-220

[10] New oblongolides isolated from the endophytic fungus Phomopsis sp from Melilotus dentata from the shores of the Baltic Sea [J].

Dai, JQ ;

Krohn, K ;

Gehle, D ;

Kock, I ;

Flörke, U ;

Aust, HJ ;

Draeger, S ;

Schulz, B ;

Rheinheimer, J .

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2005, 2005 (18) :4009-4016

← 1 2 3 4 5 →