Optical Control of CRISPR/Cas9 Gene Editing

被引：242

作者：

Hemphill, James ^{[1
]}

Borchardt, Erin K. ^{[2
,3
,4
]}

Brown, Kalyn ^{[1
]}

Asokan, Aravind ^{[2
,3
,4
]}

Deiters, Alexander ^{[1
]}

机构：

[1] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA

[2] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA

[3] Univ N Carolina, Dept Biochem, Chapel Hill, NC 27599 USA

[4] Univ N Carolina, Dept Biophys, Chapel Hill, NC 27599 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2015年 / 137卷 / 17期

基金：

美国安德鲁·梅隆基金会;

关键词：

RNA; CAS9; TRANSCRIPTION; DROSOPHILA; SYSTEM; CODE;

D O I：

10.1021/ja512664v

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The CRISPR/Cas9 system has emerged as an important tool in biomedical research for a wide range of applications, with significant potential for genome engineering and gene therapy. In order to achieve conditional control of the CRISPR/Cas9 system, a genetically encoded light-activated Cas9 was engineered through the site-specific installation of a caged lysine amino acid. Several potential lysine residues were identified as viable caging sites that can be modified to optically control Cas9 function, as demonstrated through Optical activation and deactivation of both exogenous and endogenous gene function.

引用

收藏

页码：5642 / 5645

页数：4

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