Expression of nuclear receptor mRNA and liver X receptor-mediated regulation of ABC transporter A1 at rat blood-brain barrier

被引：40

作者：

Akanuma, Shin-Ichi ^{[1
]}

Hori, Satoko ^{[1
,2
,3
]}

Ohtsuki, Sumio ^{[1
,2
,3
]}

Fujiyoshi, Masachika ^{[1
]}

Terasaki, Tetsuya ^{[1
,2
,3
]}

机构：

[1] Tohoku Univ, Div Membrane Transport & Drug Targeting, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Japan Sci & Technol Agcy, CREST, Tokyo, Japan

[3] Japan Sci & Technol Agcy, SORST, Tokyo, Japan

来源：

NEUROCHEMISTRY INTERNATIONAL | 2008年 / 52卷 / 4-5期

基金：

日本学术振兴会;

关键词：

ABC transporter; blood-brain barrier; brain capillary endothelial cell; nuclear receptor; LXR; PPAR; PXR;

D O I：

10.1016/j.neuint.2007.08.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to investigate the expression of nuclear receptor mRNA and regulation of the expression of ATP-binding cassette (ABC) transporters by nuclear receptor agonists in rat brain capillary endothelial cells, which form the blood-brain barrier, by using rat brain capillary fraction from 8-week-old rats and a conditionally immortalized brain capillary endothelial cell line (TR-BBB 13). RT-PCR analysis revealed that liver X receptor alpha and beta, retinoid X receptor alpha and beta and peroxisome proliferator-activating receptor alpha and beta mRNAs were expressed in the rat brain capillary endothelial cells and TR-BBB cells. In contrast, pregnane X receptor, famesoid X receptor and constitutive androstane receptor were not detected. Furthermore, treatment with a liver X receptor agonist increased the ABCA1 mRNA level in TR-BBB 13 cells, while ABCG2 mRNA expression was not affected. Treatment with a rat pregnane X receptor agonist did not affect the ABCB1 mRNA level in TR-BBB 13 cells. These results demonstrate that the rat blood-brain barrier has an expressional regulation mechanism via sterol-related nuclear receptor, and indicate that the blood-brain barrier in 8-week-old rats lacks ABCB1 regulation via pregnane X receptor. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：669 / 674

页数：6

共 39 条

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