Multipotency and growth characteristic of periosteum-derived progenitor cells for chondrogenic, osteogenic, and adipogenic differentiation

被引：95

作者：

Choi, Yong-Soo ^{[1
]}

Noh, Sang-Eun ^{[1
]}

Lim, Sang-Min ^{[2
]}

Lee, Chang-Woo ^{[3
]}

Kim, Chul-Soo ^{[4
]}

Im, Moon-Whan ^{[5
]}

Lee, Moon-Hee ^{[4
]}

Kim, Dong-Il ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[2] Boryung Pharmacet Co, Ansan 425120, GyeongiGi, South Korea

[3] Good Shepherd Hosp, Seoul 135717, South Korea

[4] Inha Univ, Div Hematol Oncol, Inchon 400711, South Korea

[5] Inha Univ, Dept Obstet & Gynecol, Inchon 400711, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 04期

关键词：

cell therapy; mesenchymal stem cells; multipotency; periosteum-derived progenitor cells;

D O I：

10.1007/s10529-007-9584-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The mesengenic multipotency of cryopreserved periosteum-derived progenitor cells (PDPCs) for chondrogenesis, osteogenesis and adipogenesis was investigated. Differentiation was verified using RT-PCR and histological analysis. For characterization, FACS analysis was performed with specific surface markers of mesenchymal stem cells (MSCs). Among PDPCs, unsorted periosteum-derived cells (PDCs) and dermal fibroblasts, the most distinct characteristics were found to be CD9, CD105, and CD166. In addition, these markers in PDPCs were continuously maintained until passage 15. We developed a rapid method for the isolation of PDPCs that can differentiate into mesodermal lineages and provide enough cells in a short period of time for allogeneic cell therapy.

引用

页码：593 / 601

页数：9

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