学术探索
学术期刊
学术作者
新闻热点
数据分析
智能评审

Chondrogenesis of human periosteum-derived progenitor cells in atelocollagen

被引：35
作者：
Choi, Yong-Soo
Lim, Sang-Min
Shin, Hyun-Chong
Lee, Chang-Woo
Kim, Sang-Lin
Kim, Dong-Il [1 ]
机构：
[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea
[2] Good Shepherd Hosp, Seoul 135717, South Korea
[3] Boryung Pharmaceut Co Ltd, Ansan 425120, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 02期
关键词：
atelocollagen; cartilage; chondrogenesis; periosteum-derived progenitor cells;
D O I：
10.1007/s10529-006-9240-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要：
Periosteum-derived progenitor cells (PDPCs) could be differentiated into cartilage using atelocollagen as a carrier and in the presence of transforming growth factor-b3 (TGF-b3). Chondrogenesis was verified by RT-PCR and Western blotting. Expression of the type II collagen mRNA was found from the differentiated PDPCs in atelocollagen 3 weeks after chondrogenic induction. The chondrogenic potential of the PDPCs was also verified by histochemical staining for type II collagen protein. Increased production of glycosaminoglycan shows that the PDPCs in atelocollagen could differentiate into chondrocytes under a chondrogenic environment. PDPCs can therefore be used as a cell source for cell-based therapies targeted toward the articular cartilage of the knee.
引用
收藏
页码：323 / 329
页数：7
相关论文
共 19 条
[1]
TREATMENT OF DEEP CARTILAGE DEFECTS IN THE KNEE WITH AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].
BRITTBERG, M ;
LINDAHL, A ;
NILSSON, A ;
OHLSSON, C ;
ISAKSSON, O ;
PETERSON, L .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (14) :889-895
[2]
De Bari C, 2001, ARTHRITIS RHEUM, V44, P85, DOI 10.1002/1529-0131(200101)44:1<85::AID-ANR12>3.0.CO
[3]
2-6
[4]
De Bari C, 2001, ARTHRITIS RHEUM-US, V44, P1928, DOI 10.1002/1529-0131(200108)44:8<1928::AID-ART331>3.0.CO
[5]
2-P
[6]
Identification, quantification and isolation of mesenchymal progenitor cells from osteoarthritic synovium by fluorescence automated cell sorting [J].
Fickert, S ;
Fiedler, J ;
Brenner, RE .
OSTEOARTHRITIS AND CARTILAGE, 2003, 11 (11) :790-800
[7]
Localization of chondrocyte precursors in periosteum [J].
Ito, Y ;
Fitzsimmons, JS ;
Sanyal, A ;
Mello, MA ;
Mukherjee, N ;
O'Driscoll, SW .
OSTEOARTHRITIS AND CARTILAGE, 2001, 9 (03) :215-223
[8]
Effects of cell density on proliferation and matrix synthesis of chondrocytes embedded in atelocollagen gel [J].
Iwasa, J ;
Ochi, M ;
Uchio, Y ;
Katsube, K ;
Adachi, N ;
Kawasaki, K .
ARTIFICIAL ORGANS, 2003, 27 (03) :249-255
[9]
Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis [J].
Lim, SM ;
Choi, YS ;
Shin, HC ;
Lee, CW ;
Kim, DI .
BIOTECHNOLOGY LETTERS, 2005, 27 (09) :607-611
[10]
Antigenicity and immunogenicity of collagen [J].
Lynn, AK ;
Yannas, IV ;
Bonfield, W .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2004, 71B (02) :343-354
12