Spectroelectrochemistry of cytochrome P450cam

被引：26

作者：

Bistolas, N

Christenson, A

Ruzgas, T

Jung, C

Scheller, FW

Wollenberger, U

机构：

[1] Univ Potsdam, Dept Analyt Biochem, D-14476 Golm, Germany

[2] Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany

[3] Lund Univ, Dept Analyt Chem, SE-221 Lund, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 314卷 / 03期

关键词：

cytochrome P450cam; spectroelectrochemsitry; dithionite; modified electrode;

D O I：

10.1016/j.bbrc.2003.12.159

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The spectroelectrochemistry of camphor-bound cytochrome P450cam (P450cam) using gold electrodes is described. The electrodes were modified with either 4,4'-dithiodipyridin or sodium dithionite. Electrolysis of P450cam was carried out when the enzyme was in solution, while at the same time UV visible absorption spectra were recorded. Reversible oxidation and reduction could be observed with both 4,4'-dithiodipyridin and dithionite modified electrodes. A formal potential (E-0') of -373 mV vs Ag/AgCl 1 M KCl was determined. The spectra of P450cam complexed with either carbon monoxide or metyrapone, both being inhibitors of P450 catalysis, clearly indicated that the protein retained its native state in the electrochemical cell during electrolysis. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：810 / 816

页数：7

共 40 条

[1]

Electrochemistry of P450cin:: new insights into P450 electron transfer [J].