Butane and propane oxidation by engineered cytochrome P450cam

被引：58

作者：

Bell, SG ^{[1
]}

Stevenson, JA ^{[1
]}

Boyd, HD ^{[1
]}

Campbell, S ^{[1
]}

Riddle, AD ^{[1
]}

Orton, EL ^{[1
]}

Wong, LL ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Inorgan Chem Lab, Oxford OX1 3QR, England

来源：

CHEMICAL COMMUNICATIONS | 2002年 / 05期

关键词：

D O I：

10.1039/b110957j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The haem monooxygenase cytochrome P450(cam) has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidises butane with a turnover rate of 750 min(-1) and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min(-1) with 4% yield.

引用

页码：490 / 491

页数：2

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