A novel anti-tumor agent, polyoxomolybdate induces apoptotic cell death in AsPC-1 human pancreatic cancer cells

被引：71

作者：

Ogata, A

Mitsui, S

Yanagie, H

Kasano, H

Hisa, T

Yamase, T

Eriguchi, M

机构：

[1] Univ Tokyo, Adv Sci & Technol Res Ctr, Dept Intellectual Property, Project Canc Metastasis Inhibit,Meguro Ku, Tokyo 1538904, Japan

[2] Tokyo Inst Technol, Chem Resources Lab, Midori Ku, Yokohama, Kanagawa 2268503, Japan

[3] Polytron Co Ltd, Kanazawa, Ishikawa 9200967, Japan

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2005年 / 59卷 / 05期

基金：

日本科学技术振兴机构;

关键词：

polyoxomolybdate; anti-tumor; apoptosis;

D O I：

10.1016/j.biopha.2004.11.008

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Anti-tumoral polyoxomolybdates have been investigated in the course of study of the medical application of polyoxometalates as discrete cluster anions of metal oxides. [NH3Pi](6)[Mo7O24]center dot 3H(2)O (PM-8) has been recognized as one of significantly anti-tumoral polyoxomolybdates. PM-8 inhibited the cell growth of human pancreatic cells (AsPC-1) depending on the dose. DNA ladder formation and DNA fragmentation were observed by Hoechst and TUNEL staining and flowcytometry analysis. The ratio of apoptotic cells were 29%, 35%, and 57% with treatment of PM-8 after 24, 48, and 72 h, respectively, which suggested that the anti-tumor activity of PM-8 results from the activation of the apoptotic pathway. Polyoxomolybdates provide promising, novel anti-tumor agent, especially for the treatment of cancers that are difficult to treat. (c) 2005 Elsevier SAS. All rights reserved.

引用

页码：240 / 244

页数：5

共 28 条

[11] Isolation and characterization of NMSO3-resistant mutants of respiratory syncytial virus [J].

Kimura, K ;

Ishioka, K ;

Hashimoto, K ;

Mori, S ;

Suzutani, T ;

Bowlin, TL ;

Shigeta, S .

ANTIVIRAL RESEARCH, 2004, 61 (03) :165-171

[12]

Li Z, 2004, WORLD J GASTROENTERO, V10, P1876

[13] HIGH-DOSE FOLINIC ACID, 5-FLUOROURACIL BOLUS AND INFUSION IN ADVANCED PANCREATIC ADENOCARCINOMA - A PILOT-STUDY [J].

LOUVET, C ;

BEERBLOCK, K ;

DEGRAMONT, A ;

VARETTE, C ;

DEMUYNCK, B ;

BENNAMOUN, M ;

CADY, J ;

DELFAU, S ;

MAISANI, JE ;

KRULIK, M .

EUROPEAN JOURNAL OF CANCER, 1993, 29A (08) :1217-1217

[14]

Nose H, 1999, HEPATO-GASTROENTEROL, V46, P3244

[15]

PAZDUR R, 1992, CANCER, V70, P2073, DOI 10.1002/1097-0142(19921015)70:8<2073::AID-CNCR2820700810>3.0.CO

[16]

2-A

[17] AN IMPROVED COLORIMETRIC ASSAY FOR CELL-PROLIFERATION AND VIABILITY UTILIZING THE TETRAZOLIUM SALT XTT [J].

ROEHM, NW ;

RODGERS, GH ;

HATFIELD, SM ;

GLASEBROOK, AL .

JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 142 (02) :257-265

[18] PHASE-II STUDY OF CISPLATIN AND 120-HOUR CONTINUOUS-INFUSION OF 5-FLUOROURACIL IN PATIENTS WITH ADVANCED PANCREATIC ADENOCARCINOMA [J].

ROUGIER, P ;

ZARBA, JJ ;

DUCREUX, M ;

BASILE, M ;

PIGNON, JP ;

MAHJOUBI, M ;

BENAHMED, M ;

DROZ, JP ;

CVITKOVIC, E ;

ARMAND, JP .

ANNALS OF ONCOLOGY, 1993, 4 (04) :333-336

[19] Mechanism of polyoxometalate-mediated inactivation of DNA polymerases: An analysis with HIV-1 reverse transcriptase indicates specificity for the DNA-binding cleft [J].

Sarafianos, SG ;

Kortz, U ;

Pope, MT ;

Modak, MJ .

BIOCHEMICAL JOURNAL, 1996, 319 :619-626

[20] Synergistic anti-influenza virus A (H1N1) activities of PM-523 (polyoxometalate) and ribavirin in vitro and in vivo [J].

Shigeta, S ;

Mori, S ;

Watanabe, J ;

Soeda, S ;

Takahashi, K ;

Yamase, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (07) :1423-1427

← 1 2 3 →