Enhanced mercury biosorption by bacterial cells with surface-displayed MerR

被引:96
作者
Bae, W
Wu, CH
Kostal, J
Mulchandani, A
Chen, W [1 ]
机构
[1] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA
[2] Univ Calif Riverside, Dept Environm Toxicol Program, Riverside, CA 92521 USA
关键词
D O I
10.1128/AEM.69.6.3176-3180.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The metalloregulatory protein MerR, which exhibits high affinity and selectivity toward mercury,. was exploited for the construction of microbial biosorbents specific for mercury removal. Whole-cell sorbents were constructed with MerR genetically engineered onto the surface of Escherichia coli cells by using an ice nucleation protein anchor. The presence of surface-exposed MerR on the engineered strains enabled sixfold-higher Hg2+ biosorption than that found in the wild-type JM109 cells. Hg2+ binding via MerR was very specific, with no observable decline even in the presence of 100-fold excess Cd2+ and Zn2+. The Hg2+ binding property of the whole-cell sorbents was also insensitive to different ionic strengths, pHs, and the presence of metal chelators. Since metalloregulatory proteins are currently available for a wide variety of toxic heavy metals, our results suggest that microbial biosorbents overexpressing metalloregulatory proteins may be used similarly for the cleanup of other important heavy metals.
引用
收藏
页码:3176 / 3180
页数:5
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