CsRn1, a novel active retrotransposon in a parasitic trematode, Clonorchis sinensis, discloses a new phylogenetic clade of Ty3/gypsy-like LTR retrotransposons

被引:44
作者
Bae, YA
Moon, SY
Kong, Y
Cho, SY
Rhyu, MG [1 ]
机构
[1] Catholic Univ Korea, Coll Med, Dept Microbiol, Seoul 137701, South Korea
[2] Sungkyunkwan Univ, Sch Med, Dept Mol Parasitol, Suwon, South Korea
关键词
Clonorchis sinensis; trematode; LTR retrotransposon; AP-PCR; RAPD; master copy;
D O I
10.1093/oxfordjournals.molbev.a003933
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We screened the genome of a trematode, Clonorchis sinensis, in order to identify novel retrotransposons and thereby provide additional information on retrotransposons for comprehensive phylogenetic study. Considering the vast potential of retrotransposons to generate genetically variable regions among individual genomes, randomly amplified polymorphic DNAs (RAPDs) detected by arbitrarily primed polymerase chain reactions were selected as candidates for retrotransposon-related sequences. From RAPD analysis, we isolated and characterized a novel retrotransposon in C. sinensis as the first member of uncorrupted long-terminal-repeat (LTR) retrotransposons in phylum Platyhelminthes. The retrotransposon, which was named Clonorchis sinensis Retrotransposon 1 (CsRn1), showed a genomewide distribution and had a copy number of more than 100 per haploid genome. CsRn1 encoded an uninterrupted open reading frame (ORF) of 1,304 amino acids, and the deduced ORF exhibited similarities to the pol proteins of Ty3/gypsy-like LTR retrotransposons. The mobile activity of master copies was predicted by sequence analysis and confirmed by the presence of mRNA transcripts. Phylogenetic analysis of Ty3/gypsy-like LTR retrotransposons detected a new clade comprising CsRn1, Kabuki of Bombyx mori, and an uncharacterized element of Drosophila melanogaster. With its high repetitiveness and preserved mobile activity, it is proposed that CsRn1 may play a significant role in the genomic evolution of C. sinensis.
引用
收藏
页码:1474 / 1483
页数:10
相关论文
共 42 条
[1]   Identification of novel random amplified polymorphic DNAs (RAPDs) on the W chromosome of the domesticated silkworm, Bombyx mori, and the wild silkworm, B-mandarina, and their retrotransposable element-related nucleotide sequences [J].
Abe, H ;
Kanehara, M ;
Terada, T ;
Ohbayashi, F ;
Shimada, T ;
Kawai, S ;
Suzuki, M ;
Sugasaki, T ;
Oshiki, T .
GENES & GENETIC SYSTEMS, 1998, 73 (04) :243-254
[2]   Molecular structure of a novel gypsy-Ty3-like retrotransposon (Kabuki) and nested retrotransposable elements on the W chromosome of the silkworm Bombyx mori [J].
Abe, H ;
Ohbayashi, F ;
Shimada, T ;
Sugasaki, T ;
Kawai, S ;
Mita, K ;
Oshiki, T .
MOLECULAR AND GENERAL GENETICS, 2000, 263 (06) :916-924
[3]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[4]   Transposable elements in sexual and ancient asexual taxa [J].
Arkhipova, I ;
Meselson, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) :14473-14477
[5]   Moose, a new family of LTR-retrotransposons in the mosquito Anopheles gambiae [J].
Biessmann, H ;
Walter, MF ;
Le, D ;
Chuan, S ;
Yao, JG .
INSECT MOLECULAR BIOLOGY, 1999, 8 (02) :201-212
[6]  
Boeke J. D., 1997, P343
[7]   TY ELEMENTS TRANSPOSE THROUGH AN RNA INTERMEDIATE [J].
BOEKE, JD ;
GARFINKEL, DJ ;
STYLES, CA ;
FINK, GR .
CELL, 1985, 40 (03) :491-500
[8]   L1 (LINE-1) retrotransposon evolution and amplification in recent human history [J].
Boissinot, S ;
Chevret, P ;
Furano, AV .
MOLECULAR BIOLOGY AND EVOLUTION, 2000, 17 (06) :915-928
[9]   Genomic analysis of Caenorhabditis elegans reveals ancient families of retroviral-like elements [J].
Bowen, NJ ;
McDonald, JF .
GENOME RESEARCH, 1999, 9 (10) :924-935
[10]   GYPSY/TY3-CLASS RETROTRANSPOSONS INTEGRATED IN THE DNA OF HERRING, TUNICATE, AND ECHINODERMS [J].
BRITTEN, RJ ;
MCCORMACK, TJ ;
MEARS, TL ;
DAVIDSON, EH .
JOURNAL OF MOLECULAR EVOLUTION, 1995, 40 (01) :13-24