Transforming growth factor-β1 induces epithelial-to-mesenchymal transition in human lung cancer cells via PI3K/Akt and MEK/Erk1/2 signaling pathways

被引：218

作者：

Chen, Xiao-Feng ^{[1
]}

Zhang, Hui-Jun ^{[1
,2
]}

Wang, Hai-Bing ^{[1
]}

Zhu, Jun ^{[4
]}

Zhou, Wen-Yong ^{[1
]}

Zhang, Hui ^{[1
]}

Zhao, Ming-Chuan ^{[1
]}

Su, Jin-Mei ^{[3
]}

Gao, Wen ^{[1
]}

Zhang, Lei ^{[1
]}

Fei, Ke ^{[2
]}

Zhang, Hong-Tao ^{[5
]}

Wang, He-Yong ^{[6
]}

机构：

[1] Tongji Univ, Dept Thorac Surgury, Shanghai Pulm Hosp, Shanghai 200092, Peoples R China

[2] Tongji Univ, Dept Thorac Surgury, Peoples Hosp 10, Shanghai 200092, Peoples R China

[3] Tongji Univ, Dept Integrated Chinese Med & Western Med, Shanghai Pulm Hosp, Shanghai 200092, Peoples R China

[4] Forth Peoples Hosp Wuxi, Dept Thorac Surgury, Wuxi, Jiangsu, Peoples R China

[5] Soochow Univ, Lab Canc Mol Genet, Coll Med, Suzhou, Peoples R China

[6] Tongji Univ, Cent Lab, Shanghai Pulm Hosp, Shanghai 200092, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 04期

基金：

美国国家科学基金会;

关键词：

TGF-beta; 1; EMT; A549; PI3K/Akt; MAPK/Erk1/2; GROWTH-FACTOR-BETA; DEPENDENT MECHANISM; TUMOR PROGRESSION; BREAST-CANCER; METASTASIS; TRANSDIFFERENTIATION; TGF-BETA-1; RAS; OSTEOPONTIN; DISSECTION;

D O I：

10.1007/s11033-011-1128-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Metastasis of tumor cells is associated with epithelial-to-mesenchymal transition (EMT), which is a process whereby epithelial cells lose their polarity and acquire new features of mesenchyme. EMT has been reported to be induced by transforming growth factor-beta 1 (TGF-beta 1), but its mechanism remains elusive. In this study, we performed a study to investigate whether PI3K/Akt and MAPK/Erk1/2 signaling pathways involved in EMT in the human lung cancer A549 cells. The results showed that after treated with TGF-beta 1 for 48 h, A549 cells displayed more fibroblast-like shape, lost epithelial marker E-cadherin and increased mesenchymal markers Vimentin and Fibronectin. Moreover, TGF-beta 1-induced EMT after 48 h was accompanied by increased of cell migration and change of Akt and Erk1/2 phosphorylation. In addition, EMT was reversed by PI3K inhibitor LY294002 and MEK1/2 inhibitor U0126, which suggested that A549 cells under stimulation of TGF-beta 1 undergo a switch into mesenchymal cells and PI3K/Akt and MAPK/Erk1/2 signaling pathways serve to regulate TGF-beta 1-induced EMT of A549 cells.

引用

页码：3549 / 3556

页数：8

共 41 条

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