Optimization of the determination of tocopherols in Agaricus sp edible mushrooms by a normal phase liquid chromatographic method

被引：47

作者：

Barros, Lillian ^{[1
,2
]}

Correia, Daniela M. ^{[1
]}

Ferreira, Isabel C. F. R. ^{[1
]}

Baptista, Paula ^{[1
]}

Santos-Buelga, Celestino ^{[2
]}

机构：

[1] Inst Politecn Braganca, Escola Super Agr, CIMO, P-5301855 Braganca, Portugal

[2] Univ Salamanca, Fac Farm, Unit Nutr & Bromatol, Fac Farm, E-37007 Salamanca, Spain

来源：

FOOD CHEMISTRY | 2008年 / 110卷 / 04期

关键词：

vitamin E; HPLC; analysis; mushrooms;

D O I：

10.1016/j.foodchem.2008.03.016

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid-liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1046 / 1050

页数：5

共 30 条

[21] Validation for chromatographic and electrophoretic methods [J].

Ribani, M ;

Bottoli, CBG ;

Collins, CH ;

Jardim, ICSF ;

Melo, LFC .

QUIMICA NOVA, 2004, 27 (05) :771-780

[22] A small-scale sample preparation method with HPLC analysis for determination of tocopherols and tocotrienols in cereals [J].

Ryynänen, M ;

Lampi, AM ;

Salo-Väänänen, P ;

Ollilainen, V ;

Piironen, V .

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2004, 17 (06) :749-765

[23] Determination of carotene, tocopherols and tocotrienols in residue oil from palm pressed fiber using pressurized liquid extraction-normal phase liquid chromatography [J].

Sanagi, MM ;

See, HH ;

Ibrahim, WAW ;

Abu Naim, A .

ANALYTICA CHIMICA ACTA, 2005, 538 (1-2) :71-76

[24] Does lack of tocopherols and tocotrienols put women at increased risk of breast cancer? [J].

Schwenke, DC .

JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2002, 13 (01) :2-20

[25] EPIDEMIOLOGIC EVIDENCE FOR VITAMIN-E IN PREVENTION OF CARDIOVASCULAR-DISEASE [J].

STAMPFER, MJ ;

RIMM, EB .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 1995, 62 (06) :1365-1369

[26] Tocopherols and the etiology of colon cancer [J].

Stone, WL ;

Papas, AM .

JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1997, 89 (14) :1006-1014

[27] Tocotrienol: A review of its therapeutic potential [J].

Theriault, A ;

Chao, JT ;

Wang, Q ;

Gapor, A ;

Adeli, K .

CLINICAL BIOCHEMISTRY, 1999, 32 (05) :309-319

[28] Antioxidant properties of Agaricus blazei, Agrocybe cylindracea, and Boletus edulis [J].

Tsai, Shu-Yao ;

Tsai, Hui-Li ;

Mau, Jeng-Leun .

LWT-FOOD SCIENCE AND TECHNOLOGY, 2007, 40 (08) :1392-1402

[29] Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill [J].

Turkoglu, Aziz ;

Duru, Mehmet Emin ;

Mercan, Nazime ;

Kivrak, Ibrahim ;

Gezer, Kudret .

FOOD CHEMISTRY, 2007, 101 (01) :267-273

[30] Antioxidant properties of several commercial mushrooms [J].

Yang, JH ;

Lin, HC ;

Mau, JL .

FOOD CHEMISTRY, 2002, 77 (02) :229-235

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