Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned

被引：18

作者：

Deen, Gibrilla Fadlu ^{[1
]}

McDonald, Suzanne L. R. ^{[2
]}

Marrinan, Jaclyn E. ^{[2
]}

Sesay, Foday R. ^{[3
]}

Ervin, Elizabeth ^{[4
]}

Thorson, Anna E. ^{[5
]}

Xu, Wenbo ^{[6
,7
]}

Stroher, Ute ^{[4
]}

Ongpin, Patricia ^{[8
]}

Abad, Neetu ^{[9
]}

Ariyarajah, Archchun ^{[2
]}

Malik, Tasneem ^{[10
]}

Liu, Hongtu ^{[6
,7
]}

Ross, Christine ^{[11
]}

Durski, Kara N. ^{[12
]}

Gaillard, Philippe ^{[2
]}

Morgan, Oliver ^{[13
]}

Formenty, Pierre ^{[12
]}

Knust, Barbara ^{[4
]}

Broutet, Nathalie ^{[5
]}

Sahr, Foday ^{[3
]}

机构：

[1] Connaught Hosp, Clin Studies, Internal Med, Minist Hlth & Sanitat, Freetown, Sierra Leone

[2] WHO, EVD Res, Comprehens Care & Support EVD Survivors, Freetown, Sierra Leone

[3] 34 Mil Hosp, Sierra Leone Minist Def, Freetown, Sierra Leone

[4] Ctr Dis Control & Prevent, Viral Special Pathogens Branch, Div High Consequence Pathogens & Pathol, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA USA

[5] WHO, Dept Reprod Hlth & Res Family Womens & Childrens, Geneva, Switzerland

[6] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China

[7] Chinese Ctr Dis Control & Prevent, Sierra Leone China Friendship Biol Safety Lab, Freetown, Sierra Leone

[8] Joint United Nations Programme HIV AIDS, Strateg Informat, Freetown, Sierra Leone

[9] Ctr Dis Control & Prevent, Social & Behav Res & Evaluat Branch, Div Sexually Transmitted Dis Prevent, Natl Ctr HIV AIDS Viral Hepatitis STD & TB Preven, Atlanta, GA USA

[10] Ctr Dis Control & Prevent, Off Global Act, Div Sexually Transmitted Dis Prevent, Natl Ctr HIV AIDS Viral Hepatitis STD & TB Preven, Atlanta, GA USA

[11] Ctr Dis Control & Prevent, HIV Care & Treatment Branch, Div Global HIV AIDS & TB, Ctr Global Hlth, Atlanta, GA USA

[12] WHO, Pandem & Epidem Dis Dept, Outbreaks & Hlth Emergencies, Geneva, Switzerland

[13] Ctr Dis Control & Prevent, Div Global Hlth Protect, Ctr Global Hlth, Atlanta, GA USA

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2017年 / 11卷 / 09期

关键词：

HUMAN-IMMUNODEFICIENCY-VIRUS; SEXUAL TRANSMISSION; SEMEN; HIV-1; MEN;

D O I：

10.1371/journal.pntd.0005723

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background The 2013-2016 West African Ebola virus disease epidemic was unprecedented in terms of the number of cases and survivors. Prior to this epidemic there was limited data available on the persistence of Ebola virus in survivors' body fluids and the potential risk of transmission, including sexual transmission. Methodology/Principal findings Given the urgent need to determine the persistence of Ebola virus in survivors' body fluids, an observational cohort study was designed and implemented during the epidemic response operation in Sierra Leone. This publication describes study implementation methodology and the key lessons learned. Challenges encountered during implementation included unforeseen duration of follow-up, complexity of interpreting and communicating laboratory results to survivors, and the urgency of translating research findings into public health practice. Strong community engagement helped rapidly implement the study during the epidemic. The study was conducted in two phases. The first phase was initiated within five months of initial protocol discussions and assessed persistence of Ebola virus in semen of 100 adult men. The second phase assessed the persistence of virus in multiple body fluids (semen or vaginal fluid, menstrual blood, breast milk, and urine, rectal fluid, sweat, saliva, tears), of 120 men and 120 women. Conclusion/Significance Data from this study informed national and global guidelines in real time and demonstrated the need to implement semen testing programs among Ebola virus disease survivors. The lessons learned and study tools developed accelerated the implementation of such programs in Ebola virus disease affected countries, and also informed studies examining persistence of Zika virus. Research is a vital component of the public health response to an epidemic of a poorly characterized disease. Adequate resources should be rapidly made available to answer critical research questions, in order to better inform response efforts.

引用

页数：18

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