Effect of mechanical properties of hydrogel nanoparticles on macrophage cell uptake

被引：215

作者：

Banquy, Xavier ^{[1
]}

Suarez, Fernando ^{[2
]}

Argaw, Anteneh ^{[3
]}

Rabanel, Jean-Michel ^{[1
]}

Grutter, Peter ^{[2
]}

Bouchard, Jean-Francois ^{[3
]}

Hildgen, Patrice ^{[1
,2
]}

Giasson, Suzanne ^{[1
,4
,5
,6
]}

机构：

[1] Univ Montreal, Fac Pharm, Montreal, PQ H3C 3J7, Canada

[2] McGill Univ, Dept Phys, Montreal, PQ H3A 2T8, Canada

[3] Univ Montreal, Ecole Optometrie, Montreal, PQ H3C 3J7, Canada

[4] Univ Montreal, Grp Rech Univ Medicament, Montreal, PQ H3C 3J7, Canada

[5] Univ Montreal, Dept Chem, Montreal, PQ H3C 3J7, Canada

[6] McGill Univ, Ctr Self Assembled Chem Struct, Montreal, PQ H3A 2K6, Canada

来源：

SOFT MATTER | 2009年 / 5卷 / 20期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

SINGLE LIVING CELLS; OPTICAL NANOSENSORS; CHEMICAL-ANALYSIS; COATED PIT; CLATHRIN; DELIVERY; MONOLAYERS; PATHWAYS; MICA; MACROPINOCYTOSIS;

D O I：

10.1039/b821583a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Uptake and intracellular trafficking of hydrogel nanoparticles (NPs) of N,N-diethyl acrylamide and 2-hydroxyethyl methacrylate crosslinked with N,N'-methylene-bis-acrylamide were studied with a RAW 264.7 murine macrophage cell line. Results show that the uptake rate, the mechanism of internalization and the concentration of internalized NPs are correlated to the NP Young modulus. Soft NPs are found to be internalized preferentially via macropinocytosis while the uptake of stiff NPs is mediated by a clathrin-dependent mechanism. NPs with an intermediate Young modulus exhibit multiple uptake mechanisms. The accumulation rate of the NPs into lysosomal compartments of the cell is also dependent on the NP elasticity. Our results suggest that control over the mechanical properties of hydrogel NPs can be used to tailor the cellular uptake mechanism and kinetics of drug delivery.

引用

页码：3984 / 3991

页数：8

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