Microbial lipid production from SPORL-pretreated Douglas fir by Mortierella isabellina

被引：34

作者：

Harde, S. M. ^{[1
]}

Wang, Z. ^{[1
]}

Horne, M. ^{[1
]}

Zhu, J. Y. ^{[1
,2
]}

Pan, X. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA

[2] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA

来源：

FUEL | 2016年 / 175卷

关键词：

Forest residue; Microbial lipid; SPORL pretreatment; Mortierella isabellina; Detoxification; ENHANCE ENZYMATIC SACCHARIFICATION; OLEAGINOUS YEAST; OIL PRODUCTION; CORN STOVER; YARROWIA-LIPOLYTICA; ETHANOL-PRODUCTION; FOREST RESIDUE; HIGH-TITER; ACID; FERMENTATION;

D O I：

10.1016/j.fuel.2016.02.023

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

080707 [能源环境工程]; 082001 [油气井工程];

摘要：

The solid substrate and spent liquor obtained after SPORL pretreatment of Douglas fir residues were evaluated as substrates for the production of intracellular microbial lipid by Mortierella isabellina NRRL 1757. The production of lipid was investigated in a batch fermentation using different strategies viz. separate hydrolysis and fermentation, quasi-simultaneous saccharification and fermentation (Q-SSF), and whole slurry (solid substrate and spent liquor) saccharification and fermentation. The maximum lipid yield (0.21 g/g sugar) was obtained with Q-SSF of the solid substrate. Nutrient supplementation was not necessary for lipid production from SPORL-pretreated Douglas fir. Detoxification of SPORL spent liquor with lime did remove the inhibitors and improved the fermentation for lipid production. The lipid produced had a similar fatty acid compositional profile to those of vegetable oil, suggesting that it could have potential as a substrate for biodiesel production. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：64 / 74

页数：11

共 62 条

[1]

BARNETT D, 1985, FOOD TECHNOL AUST, V37, P503

[2]

Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi [J].

Chatzifragkou, Afroditi ;

Fakas, Stylianos ;

Galiotou-Panayotou, Maria ;

Komaitis, Michael ;

Aggelis, George ;

Papanikolaou, Seraphim .

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 2010, 112 (09) :1048-1057

[3]

Screening of Oleaginous Yeast Strains Tolerant to Lignocellulose Degradation Compounds [J].

Chen, Xi ;

Li, Zihui ;

Zhang, Xiaoxi ;

Hu, Fengxian ;

Ryu, Dewey D. Y. ;

Bao, Jie .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 159 (03) :591-604

[4]

High titer and yield ethanol production from undetoxified whole slurry of Douglas-fir forest residue using pH profiling in SPORL [J].

Cheng, Jinlan ;

Leu, Shao-Yuan ;

Zhu, J. Y. ;

Gleisner, Rolland .

BIOTECHNOLOGY FOR BIOFUELS, 2015, 8

[5]

Cohen Z., 2005, Single Cell Oils, DOI 10.1201/9781439822364

[6]

The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rhodotorula glutinis [J].

Easterling, Emily R. ;

French, W. Todd ;

Hernandez, Rafael ;

Licha, Margarita .

BIORESOURCE TECHNOLOGY, 2009, 100 (01) :356-361

[7]

Modeling of Single-Cell Oil Production Under Nitrogen-Limited and Substrate Inhibition Conditions [J].

Economou, C. N. ;

Aggelis, G. ;

Pavlou, S. ;

Vayenas, D. V. .

BIOTECHNOLOGY AND BIOENGINEERING, 2011, 108 (05) :1049-1055

[8]

Single cell oil production from rice hulls hydrolysate [J].

Economou, Ch. N. ;

Aggelis, G. ;

Pavlou, S. ;

Vayenas, D. V. .

BIORESOURCE TECHNOLOGY, 2011, 102 (20) :9737-9742

[9]

Semi-solid state fermentation of sweet sorghum for the biotechnological production of single cell oil [J].

Economou, Ch. N. ;

Makri, A. ;

Aggelis, G. ;

Pavlou, S. ;

Vayenas, D. V. .

BIORESOURCE TECHNOLOGY, 2010, 101 (04) :1385-1388

[10]

Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina [J].

Fakas, Stylianos ;

Papanikolaou, Seraphim ;

Batsos, Athanasios ;

Galiotou-Panayotou, Maria ;

Mallouchos, Athanasios ;

Aggelis, George .

BIOMASS & BIOENERGY, 2009, 33 (04) :573-580

← 1 2 3 4 5 6 7 →