Potential involvement of both type I and type II mechanisms in M13 virus inactivation by methylene blue photosensitization

被引：25

作者：

Abe, H

Ikebuchi, K

Wagner, SJ

Kuwabara, M

Kamo, N

Sekiguchi, S

机构：

[1] AMER RED CROSS,BLOOD SERV,JEROME H HOLLAND LAB,PROD DEV LAB,ROCKVILLE,MD

[2] HOKKAIDO UNIV,GRAD SCH VET MED,RADIAT BIOL LAB,SAPPORO,HOKKAIDO,JAPAN

[3] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1997年 / 66卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1997.tb08644.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the mechanism of virus photoinactivation with methylene blue (MB) by conducting deuterium oxide (D2O), azide ion (N-3(-)) and oxygen-dependent studies, Inactivation of M13 bacteriophage acid singlet oxygen (O-1(2)) generation by MB photosensitization were irradiation dose dependent, Inactivation of M13 was enhanced by D2O and inhibited by N-3(-), suggesting that O-1(2) participates in M13 inactivation by MB photosensitization. However, N-3(-) did, not inhibit M13 inactivation completely, On the other fiend, deoxygenating the reaction solution still caused 52-67% of M13 inactivation observed in the presence of oxygen, These results suggest that O-1(2)-mediated (Type II) and sensitizer-mediated (Type II) reactions may both play roles in M13 inactivation by MB photosensitization.

引用

页码：204 / 208

页数：5

共 25 条

[1] ANALYSIS OF VIRAL-DNA, PROTEIN AND ENVELOPE DAMAGE AFTER METHYLENE-BLUE, PHTHALOCYANINE DERIVATIVE OF MEROCYANINE-540 PHOTOSENSITIZATION [J].