GlycoBase and autoGU: tools for HPLC-based glycan analysis

被引：210

作者：

Campbell, Matthew P. ^{[1
]}

Royle, Louise ^{[1
]}

Radcliffe, Catherine M. ^{[1
]}

Dwek, Raymond A. ^{[1
]}

Rudd, Pauline M. ^{[1
]}

机构：

[1] Univ Oxford, Dept Biochem, Oxford Glycobiol Inst, Oxford OX1 3QU, England

来源：

BIOINFORMATICS | 2008年 / 24卷 / 09期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1093/bioinformatics/btn090

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of robust high-performance liquid chromatography (HPLC) technologies continues to improve the detailed analysis and sequencing of glycan structures released from glycoproteins. Here, we present a database (GlycoBase) and analytical tool (autoGU) to assist the interpretation and assignment of HPLC-glycan profiles. GlycoBase is a relational database which contains the HPLC elution positions for over 350 2-AB labelled N-glycan structures together with predicted products of exoglycosidase digestions. AutoGU assigns provisional structures to each integrated HPLC peak and, when used in combination with exoglycosidase digestions, progressively assigns each structure automatically based on the footprint data. These tools are potentially very promising and facilitate basic research as well as the quantitative high-throughput analysis of low concentrations of glycans released from glycoproteins. Availability: http://glycobase.ucd.ie Contact: matthew.campbell@nibrt.ie.

引用

页码：1214 / 1216

页数：3

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