Gene targeting and somatic cell genetics - a rebirth or a coming of age?

被引：95

作者：

Sedivy, JM ^{[1
]}

Dutriaux, A ^{[1
]}

机构：

[1] Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA

来源：

TRENDS IN GENETICS | 1999年 / 15卷 / 03期

关键词：

D O I：

10.1016/S0168-9525(98)01689-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A number of recent advances have significantly facilitated gene targeting in somatic cells. Gene targeting can now be performed with the same ease and efficiency in somatic cells as in murine embryonic stem cells. Rigorous genetic analyses can therefore be applied for the first time to the large number of excellent human cell culture systems. These tools wilt be important in areas where rodent models do not adequately represent human biology.

引用

页码：88 / 90

页数：3

共 22 条

[1]

Abuin A, 1996, MOL CELL BIOL, V16, P1851

[2]

[Anonymous], SCIENCE

[3] GENE TARGETING IN NORMAL SOMATIC-CELLS - INACTIVATION OF THE INTERFERON-GAMMA RECEPTOR IN MYOBLASTS [J].

ARBONES, ML ;

AUSTIN, HA ;

CAPON, DJ ;

GREENBURG, G .

NATURE GENETICS, 1994, 6 (01) :90-97

[4] Reduced X-ray resistance and homologous recombination frequencies in a RAD54(-/-) mutant of the chicken DT40 cell line [J].

Bezzubova, O ;

Silbergleit, A ;

YamaguchiIwai, Y ;

Takeda, S ;

Buerstedde, JM .

CELL, 1997, 89 (02) :185-193

[5]

BOLLAG RJ, 1989, ANNU REV GENET, V23, P199, DOI 10.1146/annurev.genet.23.1.199

[6] Bypass of senescence after disruption of p21(CIP1/WAF1) gene in normal diploid human fibroblasts [J].

Brown, JP ;

Wei, WY ;

Sedivy, JM .

SCIENCE, 1997, 277 (5327) :831-834

[7] Requirement for p53 and p21 to sustain G2 arrest after DNA damage [J].

Bunz, F ;

Dutriaux, A ;

Lengauer, C ;

Waldman, T ;

Zhou, S ;

Brown, JP ;

Sedivy, JM ;

Kinzler, KW ;

Vogelstein, B .

SCIENCE, 1998, 282 (5393) :1497-1501

[8] c-myc null cells misregulate cad and gadd45 but not other proposed c-Myc targets [J].

Bush, A ;

Mateyak, M ;

Dugan, K ;

Obaya, A ;

Adachi, S ;

Sedivy, J ;

Cole, M .

GENES & DEVELOPMENT, 1998, 12 (24) :3797-3802

[9]

Davies Monique V., 1992, Current Opinion in Biotechnology, V3, P512, DOI 10.1016/0958-1669(92)90079-X

[10] DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes [J].

Grawunder, U ;

Zimmer, D ;

Fugmann, S ;

Schwarz, K ;

Lieber, MR .

MOLECULAR CELL, 1998, 2 (04) :477-484

← 1 2 3 →