DNA repair activity in protein extracts from rat tissues

被引：17

作者：

Coudore, F ^{[1
]}

Calsou, P ^{[1
]}

Salles, B ^{[1
]}

机构：

[1] CNRS,UPR 9062,INST PHARMACOL & BIOL STRUCT,F-31077 TOULOUSE,FRANCE

来源：

FEBS LETTERS | 1997年 / 414卷 / 03期

关键词：

DNA repair; tissue;

D O I：

10.1016/S0014-5793(97)01077-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among DNA repair pathways, nucleotide excision repair (NER) is able to recognize and process a wide variety of DNA lesions, The NER mechanism can be summarized in two stages: incision/excision of the lesion and DNA repair synthesis. Here, we have assessed the repair synthesis activity of protein extracts from different rat tissues by an in vitro biochemical assay that reproduces the entire NER reaction, The protein extraction procedure was adapted to rat tissues and the biochemical parameters of the assay (high salt concentration, addition of EGTA) in order to minimize non-specific nuclease activity which allows the measurement of repair activity, Using this repair assay me detected a small increase in the extent of repair synthesis in liver compared to brain and lung tissue protein extracts, Similar results were obtained using a derivative assay that allows the measurement of the incision activity of tissue protein extracts with lower incision activity in lung tissue extract, (C) 1997 Federation of European Biochemical Societies.

引用

页码：581 / 584

页数：4

共 21 条

[1]

Barret JM, 1996, MOL PHARMACOL, V49, P766

[2]

BARRET JM, 1994, MOL PHARMACOL, V46, P431

[3]

BARRET JM, 1995, CARCINOGENESIS, V7, P1611

[4] EFFECT OF EXOGENOUS DNA FRAGMENTS ON HUMAN CELL EXTRACT-MEDIATED DNA-REPAIR SYNTHESIS [J].

BIGGERSTAFF, M ;

ROBINS, P ;

COVERLEY, D ;

WOOD, RD .

MUTATION RESEARCH, 1991, 254 (03) :217-224

[5] FORMATION AND PERSISTENCE OF BENZO[A]PYRENE DNA ADDUCTS IN DIFFERENT TISSUES OF C57BL/10 AND DBA/2 MICE [J].

BRAUZE, D ;

MIKSTACKA, R ;

BAERDUBOWSKA, W .

CARCINOGENESIS, 1991, 12 (09) :1607-1611

[6] PROPERTIES OF DAMAGE-DEPENDENT DNA INCISION BY NUCLEOTIDE EXCISION-REPAIR IN HUMAN CELL-FREE-EXTRACTS [J].

CALSOU, P ;

SALLES, B .

NUCLEIC ACIDS RESEARCH, 1994, 22 (23) :4937-4942

[7] Preferential repair incision of cross-links versus monoadducts in psoralen-damaged plasmid DNA by human cell-free extracts [J].

Calsou, P ;

Sage, E ;

Moustacchi, E ;

Salles, B .

BIOCHEMISTRY, 1996, 35 (47) :14963-14969

[8] MEASUREMENT OF DAMAGE-SPECIFIC DNA INCISION BY NUCLEOTIDE EXCISION-REPAIR IN-VITRO [J].

CALSOU, P ;

SALLES, B .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 202 (02) :788-795

[9] Role of transcription-coupled DNA repair in susceptibility to environmental carcinogenesis [J].

Hanawalt, PC .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1996, 104 :547-551

[10]

HANSSON J, 1989, NUCLEIC ACIDS RES, V20, P8073

← 1 2 3 →