Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfers

被引：106

作者：

De Vos, A. ^{[1
]}

Staessen, C. ^{[2
]}

De Rycke, M. ^{[2
]}

Verpoest, W. ^{[1
]}

Haentjens, P. ^{[3
,4
]}

Devroey, P. ^{[1
]}

Liebaers, I. ^{[2
]}

Van de Velde, H. ^{[1
]}

机构：

[1] Univ Ziekenhuis Brussel, Ctr Reprod Med, B-1090 Brussels, Belgium

[2] Univ Ziekenhuis Brussel, Ctr Med Genet, B-1090 Brussels, Belgium

[3] Vrije Univ Brussels, Ctr Outcomes Res, Univ Ziekenhuis Brussel, B-1090 Brussels, Belgium

[4] Vrije Univ Brussels, Expt Surg Lab, Univ Ziekenhuis Brussel, B-1090 Brussels, Belgium

来源：

HUMAN REPRODUCTION | 2009年 / 24卷 / 12期

关键词：

cleavage-stage embryo biopsy; single blastocyst transfer; PGD; 1-cell removal; 2-cell removal; PREIMPLANTATION GENETIC DIAGNOSIS; 2; BLASTOMERES; CYCLES; CONSORTIUM; REMOVAL; CELLS; IVF; AGE;

D O I：

10.1093/humrep/dep251

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Human embryo biopsy is performed for preimplantation genetic diagnosis (PGD). The impact of 1- or 2-cell removal at cleavage-stage on future embryo development and implantation capacity is highly debated. In order to explore this issue further, a cohort of Day 5 single embryo transfers was analysed prospectively for embryological and clinical outcome. All transferred embryos resulted from 8-cell embryos on Day 3, from which subsequently either one cell (group I, n = 182) or two cells (group II, n = 259) were removed, or on which no invasion by means of embryo biopsy was performed (group III, control group, n = 702). Blastocyst formation was significantly better in group III compared with group II, and similar to group I. Group I and group II did not differ in Day 3 nor in Day 5 embryo development. The overall live birth rate was significantly higher in group I (37.4%, CI 29.0-47.4%) than in group II (22.4%, CI 17.0-28.9%), and comparable to the reference ICSI population (35.0%, CI 30.8-39.7%). The clinical outcome of 1-cell biopsy was significantly better than that of 2-cell biopsy, even when adjusted for availability of genetically transferable embryos.

引用

页码：2988 / 2996

页数：9

共 24 条

[1] Neonatal data on a cohort of 2889 infants born after ICSI (1991-1999) and of 2995 infants born after IVF (1983-1999) [J].

Bonduelle, M ;

Liebaers, I ;

Deketelaere, V ;

Derde, MP ;

Camus, M ;

Devroey, P ;

Van Steirteghem, A .

HUMAN REPRODUCTION, 2002, 17 (03) :671-694

[2] Removal of 2 cells from cleavage stage embryos is likely to reduce the efficacy of chromosomal tests that are used to enhance implantation rates [J].

Cohen, Jacques ;

Wells, Dagan ;

Munne, Santiago .

FERTILITY AND STERILITY, 2007, 87 (03) :496-503

[3] Aspects of biopsy procedures prior to preimplantation genetic diagnosis [J].

De Vos, A ;

Van Steirteghem, A .

PRENATAL DIAGNOSIS, 2001, 21 (09) :767-780

[4] Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer [J].

Gardner, DK ;

Lane, M ;

Stevens, J ;

Schlenker, T ;

Schoolcraft, WB .

FERTILITY AND STERILITY, 2000, 73 (06) :1155-1158

[5] Diagnostic efficiency, embryonic development and clinical outcome after the biopsy of one or two blastomeres for preimplantation genetic diagnosis [J].

Goossens, Veerle ;

De Rycke, Martine ;

De Vos, Anick ;

Staessen, Catherine ;

Michiels, An ;

Verpoest, Willem ;

Van Steirteghem, Andre ;

Bertrand, Catherine ;

Liebaers, Inge ;

Devroey, Paul ;

Sermon, Karen .

HUMAN REPRODUCTION, 2008, 23 (03) :481-492

[6] HUMAN PREIMPLANTATION DEVELOPMENT INVITRO IS NOT ADVERSELY AFFECTED BY BIOPSY AT THE 8-CELL STAGE [J].

HARDY, K ;

MARTIN, KL ;

LEESE, HJ ;

WINSTON, RML ;

HANDYSIDE, AH .

HUMAN REPRODUCTION, 1990, 5 (06) :708-714

[7] ESHRE PGD consortium data collection VII: cycles from January to December 2004 with pregnancy follow-up to October 2005 [J].

Harper, J. C. ;

de Die-Smulders, C. ;

Goossens, V. ;

Harton, G. ;

Moutou, C. ;

Repping, S. ;

Scriven, P. N. ;

SenGupta, S. ;

Traeger-Synodinos, J. ;

Van Rij, M. C. ;

Viville, S. ;

Wilton, L. ;

Sermon, K. D. .

HUMAN REPRODUCTION, 2008, 23 (04) :741-755

[8] Comparison of the results of human embryo biopsy and outcome of PGD after zona drilling using acid Tyrode medium or a laser [J].

Joris, H ;

De Vos, A ;

Janssens, R ;

Devroey, P ;

Liebaers, I ;

Van Steirteghem, A .

HUMAN REPRODUCTION, 2003, 18 (09) :1896-1902

[9] Should we advise patients undergoing IVF to start a cycle leading to a day 3 or a day 5 transfer? [J].

Kolibianakis, EM ;

Zikopoulos, K ;

Verpoest, W ;

Camus, M ;

Joris, H ;

Van Steirteghem, AC ;

Devroey, P .

HUMAN REPRODUCTION, 2004, 19 (11) :2550-2554

[10] Controlling misdiagnosis errors in preimplantation genetic diagnosis:: a comprehensive model encompassing extrinsic and intrinsic sources of error [J].

Lewis, CM ;

Pinêl, T ;

Whittaker, JC ;

Handyside, AH .

HUMAN REPRODUCTION, 2001, 16 (01) :43-50

← 1 2 3 →