Gcn4 activator targets Gcn5 histone acetyltransferase to specific promoters independently of transcription

被引：162

作者：

Kuo, MH

vom Baur, E

Struhl, K

Allis, CD ^{[1
]}

机构：

[1] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[2] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[3] Univ Virginia, Hlth Sci Ctr, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA

来源：

MOLECULAR CELL | 2000年 / 6卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(00)00129-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone acetylation correlates well with transcriptional activity, and histone acetyltransferases (HATs) selectively regulate subsets of target genes by mechanisms that remain unclear. Here, we provide in vivo evidence that the yeast transcriptional activator Gcn4 recruits Gcn5 HAT complexes to selective promoters positioned in natural or ectopic locations, thereby creating local domains of histone H3 hyperacetylation and subsequent transcriptional activation. A significant portion of the Gcn4-targeted histone acetylation by Gcn5 is independent of transcriptional activity. These observations provide strong evidence for promoter-selective, targeted histone acetylation by Gcn5 that facilitates transcription in a causal fashion. In addition, Gcn5 also functions in an untargeted manner to acetylate H3 on a genome-wide scale.

引用

收藏

页码：1309 / 1320

页数：12

相关论文

共 48 条

[1] Gene regulation - Local or global? [J].

Berger, SL .

NATURE, 2000, 408 (6811) :412-415

[2] GENETIC ISOLATION OF ADA2 - A POTENTIAL TRANSCRIPTIONAL ADAPTER REQUIRED FOR FUNCTION OF CERTAIN ACIDIC ACTIVATION DOMAINS [J].

BERGER, SL ;

PINA, B ;

SILVERMAN, N ;

MARCUS, GA ;

AGAPITE, J ;

REGIER, JL ;

TRIEZENBERG, SJ ;

GUARENTE, L .

CELL, 1992, 70 (02) :251-265

[3] The many HATs of transcription coactivators [J].

Brown, CE ;

Lechner, T ;

Howe, L ;

Workman, JL .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (01) :15-19

[4] Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase [J].

Chen, HW ;

Lin, RJ ;

Xie, W ;

Wilpitz, D ;

Evans, RM .

CELL, 1999, 98 (05) :675-686

[5] SATURATION MUTAGENESIS OF A YEAST-HIS3 TATA ELEMENT - GENETIC-EVIDENCE FOR A SPECIFIC TATA-BINDING PROTEIN [J].

CHEN, W ;

STRUHL, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2691-2695

[6] CDC39, AN ESSENTIAL NUCLEAR-PROTEIN THAT NEGATIVELY REGULATES TRANSCRIPTION AND DIFFERENTIALLY AFFECTS THE CONSTITUTIVE AND INDUCIBLE HIS3 PROMOTERS [J].

COLLART, MA ;

STRUHL, K .

EMBO JOURNAL, 1993, 12 (01) :177-186

[7] NOT1(CDC39), NOT2(CDC36), NOT3, AND NOT4 ENCODE A GLOBAL-NEGATIVE REGULATOR OF TRANSCRIPTION THAT DIFFERENTIALLY AFFECTS TATA-ELEMENT UTILIZATION [J].

COLLART, MA ;

STRUHL, K .

GENES & DEVELOPMENT, 1994, 8 (05) :525-537

[8] Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter (Publication with Expression of Concern) [J].

Cosma, MP ;

Tanaka, TU ;

Nasmyth, K .

CELL, 1999, 97 (03) :299-311

[9] The Gcn4p activation domain interacts specifically in vitro with RNA polymerase II holoenzyme, TFIID, and the Adap-Gcn5p coactivator complex [J].

Drysdale, CM ;

Jackson, BM ;

McVeigh, R ;

Klebanow, ER ;

Bai, Y ;

Kokubo, T ;

Swanson, M ;

Nakatani, Y ;

Weil, PA ;

Hinnebusch, AG .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (03) :1711-1724

[10] The Spt components of SAGA facilitate TBP binding to a promoter at a post-activator-binding step in vivo [J].

Dudley, AM ;

Rougeulle, C ;

Winston, F .

GENES & DEVELOPMENT, 1999, 13 (22) :2940-2945

← 1 2 3 4 5 →