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Cloning and sequencing of the nhaB gene encoding an Na+/H+ antiporter from Vibrio alginolyticus

被引：31
作者：
Nakamura, T
Enomoto, H
Unemoto, T
机构：
[1] Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Chiba 263, 1-33 Yayoi-cho, Inage-ku
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1996年 / 1275卷 / 03期
关键词：
sodium ion/proton antiporter; nhaB gene; DNA sequence; marine bacterium; (V-alginolyticus);
D O I：
10.1016/0005-2728(96)00034-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A gene has been cloned from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli, TO114, defective in three Na+/H+ antiport genes (nhaA, nhaB, chaA). The nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 528 amino acid sequence and molecular mass of 57212 Da. This gene has 62% identity to nhaB gene at the DNA level from Escherichia coli and the deduced amino acid sequence is 67% identical with E. coli NhaB. This gene is presumably the V. alginolyticus nhaB gene and will be named nhaBv.
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收藏
页码：157 / 160
页数：4
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