SEQUENCING AND THE ALIGNMENT OF STRUCTURAL GENES IN THE NQR OPERON ENCODING THE NA+-TRANSLOCATING NADH-QUINONE REDUCTASE FROM VIBRIO-ALGINOLYTICUS

被引:67
作者
HAYASHI, M
HIRAI, K
UNEMOTO, T
机构
[1] Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba, 263
关键词
NA+ PUMP; NADH-QUINONE REDUCTASE; NUCLEOTIDE SEQUENCE; NQR OPERON; VIBRIO ALGINOLYTICUS;
D O I
10.1016/0014-5793(95)00283-F
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We previously cloned a part of nqr operon encoding the Na+-translocating NADH-quinone reductase (NQR) from the marine Vibrio alginolyticus [Hayashi et al., FEES Lett, 356 (1994) 330-332], From its nucleotide sequences, four consecutive open reading frames (ORF) encoding the gamma-subunit (27.7 kDa), two unidentified ORFs of 22.6 kDa and 21.5 kDa, and the beta-subunit (45.3 kDa) were recognized. The gene encoding the alpha-subunit was located upstream, and together with the recent report by Beattie et al. [FEES Lett, 356 (1994) 333-338], the nqr operon was found to be constructed from six consecutive structural genes, where nqr1, nqr3 and nqr6 correspond to the alpha-, gamma-, and beta-subunits, respectively, of the NQR complex.
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页码:75 / 77
页数:3
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