In planta monitoring of the activity of two constitutive promoters, CaMV 35S and TR2′, in developing feeding cells induced by Globodera rostochiensis using green fluorescent protein in combination with confocal laser scanning microscopy

被引：35

作者：

Goverse, A ^{[1
]}

Biesheuvel, J ^{[1
]}

Wijers, GJ ^{[1
]}

Gommers, FJ ^{[1
]}

Bakker, J ^{[1
]}

Schots, A ^{[1
]}

Helder, J ^{[1
]}

机构：

[1] Agr Univ Wageningen, Grad Sch Expt Plant Sci, Dept Nematol, NL-6709 PD Wageningen, Netherlands

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 1998年 / 52卷 / 04期

关键词：

D O I：

10.1006/pmpp.1998.0154

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Under the control of either the constitutive CaMV 35S or the mannopine synthase TR2' promoter, the green fluorescent protein (GFP) from the jellyfish Aeguorea victoria was expressed in transgenic potato (Solanum tuberosum) plants. Confocal laser scanning microscopy (CLSM) was applied to observe GFP in plants and, subsequently, to investigate promoter activity in developing feeding cells developed during potato cyst nematode (Globodera rostochiensis) infection. Both the CaMV 35S and the TR2' promoter were strongly upregulated in young feeding cells in less than 4 days upon infection by G. rostochiensis, whereas the GFP level in the surrounding tissues remained low. Optical sectioning revealed intense green fluorescence in the dense cytoplasm of the entire syncytial cell, including the most distal cell. Furthermore, GFP was observed within the digestive system of the feeding nematode, showing that proteins with an apparent molecular weight of 32 kDa can be taken up by parasitic juveniles of G. rostochiensis. Provided CLSM is used, GFP was shown to be a powerful tool that allows in vivo monitoring of gene expression inside young developing feeding cells. Finally, the transcriptional regulation of the CaMV 35S and TR2' promoter in plant-nematode interactions is discussed. (C) 1998 Academic Press.

引用

页码：275 / 284

页数：10

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