Molecular identification of arbuscular mycorrhizal fungi in roots: Perspectives and problems

被引:72
作者
Redecker, D [1 ]
Hijri, I [1 ]
Wiemken, A [1 ]
机构
[1] Univ Basel, Inst Bot, CH-4056 Basel, Switzerland
关键词
arbuscular mycorrhiza; internal transcribed spacers; mycorrhizal ecology; polymerase chain reaction; rDNA;
D O I
10.1007/BF02803144
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Molecular identification methods are about to revolutionize studies on ecology of arbuscular mycorrhiza. These techniques offer the unique opportunity to investigate communities of arbuscular mycorrhizal fungi (AMF) within roots. Recent technical advances are reviewed, discussing their drawbacks and advantages. An experimental approach to analyze AMF communities within roots using a molecular identification method is presented. Sample results from the analysis of trap cultures from a current project are shown.
引用
收藏
页码:113 / 124
页数:12
相关论文
共 45 条
[1]   BIOLOGICAL SPECIES OF ARMILLARIA-MELLEA IN NORTH-AMERICA [J].
ANDERSON, JB ;
ULLRICH, RC .
MYCOLOGIA, 1979, 71 (02) :402-414
[2]   Variation in rDNA ITS sequences in Glomus mosseae and Gigaspora margarita spores from a permanent pasture [J].
Antoniolli, ZI ;
Schachtman, DP ;
Ophel-Keller, K ;
Smith, SE .
MYCOLOGICAL RESEARCH, 2000, 104 :708-715
[3]   Host-dependent sporulation and species diversity of arbuscular mycorrhizal fungi in a mown grassland [J].
Bever, JD ;
Morton, JB ;
Antonovics, J ;
Schultz, PA .
JOURNAL OF ECOLOGY, 1996, 84 (01) :71-82
[4]   Epiparasitic plants specialized on arbuscular mycorrhizal fungi [J].
Bidartondo, MI ;
Redecker, D ;
Hijri, I ;
Wiemken, A ;
Bruns, TD ;
Domínguez, L ;
Sérsic, A ;
Leake, JR ;
Read, DJ .
NATURE, 2002, 419 (6905) :389-392
[5]  
Buckler ES, 1997, GENETICS, V145, P821
[6]   DIVERSITY OF FUNGAL SYMBIONTS IN ARBUSCULAR MYCORRHIZAS FROM A NATURAL COMMUNITY [J].
CLAPP, JP ;
YOUNG, JPW ;
MERRYWEATHER, JW ;
FITTER, AH .
NEW PHYTOLOGIST, 1995, 130 (02) :259-265
[7]   Inter- and intra-isolate rRNA large subunit variation in Glomus coronatum spores [J].
Clapp, JP ;
Rodriguez, A ;
Dodd, JC .
NEW PHYTOLOGIST, 2001, 149 (03) :539-554
[8]   Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. [J].
Clapp, JP ;
Fitter, AH ;
Young, JPW .
MOLECULAR ECOLOGY, 1999, 8 (06) :915-921
[9]   DETECTION OF AN ARBUSCULAR MYCORRHIZAL FUNGUS IN ROOTS OF DIFFERENT PLANT-SPECIES WITH THE PCR [J].
DIBONITO, R ;
ELLIOTT, ML ;
JARDIN, EAD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2809-2810
[10]   Inter- and intraspecific variation within the morphologically similar arbuscular mycorrhizal fungi Glomus mosseae and Glomus coronatum [J].
Dodd, JC ;
Rosendahl, S ;
Giovannetti, M ;
Broome, A ;
Lanfranco, L ;
Walker, C .
NEW PHYTOLOGIST, 1996, 133 (01) :113-122