Molecular identification of arbuscular mycorrhizal fungi in roots: Perspectives and problems

被引:72
作者
Redecker, D [1 ]
Hijri, I [1 ]
Wiemken, A [1 ]
机构
[1] Univ Basel, Inst Bot, CH-4056 Basel, Switzerland
关键词
arbuscular mycorrhiza; internal transcribed spacers; mycorrhizal ecology; polymerase chain reaction; rDNA;
D O I
10.1007/BF02803144
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Molecular identification methods are about to revolutionize studies on ecology of arbuscular mycorrhiza. These techniques offer the unique opportunity to investigate communities of arbuscular mycorrhizal fungi (AMF) within roots. Recent technical advances are reviewed, discussing their drawbacks and advantages. An experimental approach to analyze AMF communities within roots using a molecular identification method is presented. Sample results from the analysis of trap cultures from a current project are shown.
引用
收藏
页码:113 / 124
页数:12
相关论文
共 45 条
[21]  
Jansa J, 2002, MYCORRHIZA, V12, P225, DOI [10.1007/s00572-002-0163-z, 10.1007/s005572-002-0163-z]
[22]   Molecular diversity of glomalean (arbuscular mycorrhizal) fungi determined as distinct Glomus specific DNA sequences from roots of field grown peas [J].
Kjoller, R ;
Rosendahl, S .
MYCOLOGICAL RESEARCH, 2001, 105 :1027-1032
[23]   Concordance of gene genealogies reveals reproductive isolation in the pathogenic fungus Coccidioides immitis [J].
Koufopanou, V ;
Burt, A ;
Taylor, JW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (10) :5478-5482
[24]   Evidence for the evolution of multiple genomes in arbuscular mycorrhizal fungi [J].
Kuhn, G ;
Hijri, M ;
Sanders, IR .
NATURE, 2001, 414 (6865) :745-748
[25]   Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita [J].
Lanfranco, L ;
Delpero, M ;
Bonfante, P .
MOLECULAR ECOLOGY, 1999, 8 (01) :37-45
[26]   GENERATION OF RAPD-PCR PRIMERS FOR THE IDENTIFICATION OF ISOLATES OF GLOMUS-MOSSEAE, AN ARBUSCULAR MYCORRHIZAL FUNGUS [J].
LANFRANCO, L ;
WYSS, P ;
MARZACHI, C ;
BONFANTE, P .
MOLECULAR ECOLOGY, 1995, 4 (01) :61-68
[27]   Diversity of the ribosomal internal transcribed spacers within and among isolates of Glomus mosseae and related mycorrhizal fungi [J].
LloydMacgilp, SA ;
Chambers, SM ;
Dodd, JC ;
Fitter, AH ;
Walker, C ;
Young, JPW .
NEW PHYTOLOGIST, 1996, 133 (01) :103-111
[28]   The arbuscular mycorrhizal fungi of Hyacinthoides non-scripta - I. Diversity of fungal taxa [J].
Merryweather, J ;
Fitter, A .
NEW PHYTOLOGIST, 1998, 138 (01) :117-129
[29]   Two new families of Glomales, Archaeosporaceae and Paraglomaceae, with two new genera Archaeospora and Paraglomus, based on concordant molecular and morphological characters [J].
Morton, JB ;
Redecker, D .
MYCOLOGIA, 2001, 93 (01) :181-195
[30]   RIBOSOMAL DNA INTERNAL TRANSCRIBED SPACERS ARE HIGHLY DIVERGENT IN THE PHYTOPATHOGENIC ASCOMYCETE FUSARIUM-SAMBUCINUM (GIBBERELLA-PULICARIS) [J].
ODONNELL, K .
CURRENT GENETICS, 1992, 22 (03) :213-220