High-resolution genetic and physical mapping of modifier-of-deafwaddler (mdfw) and waltzer (Cdh23v)

Modifier-of-deafwaddler (mdfw) and waltzer (Cdh23(v)) are loci on mouse chromosome 10 encoding factors that are essential for the function of auditory hair cells. The BALB/cByJ-specific mdfw allele encodes a necessary and sufficient modifier that induces progressive early onset hearing loss in CBy-dfw(2J) heterozygotes. Recessive mutations in the waltzer locus result in:circling behavior and congenital deafness. In this report we present a high-resolution integrated genetic and physical map of mdfw and Cdh23(v). Our genetic analyses localize mdfw, between markers D10Mit60 and 148M13T7 within a 1.01-cM region. The Cdh23(v) critical interval is fully contained within the mdfw, region and localizes between markers 146O23T7 and 148M13T7 within a 0.35-cM interval that is represented in an approximate to 500-kb BAC contig, Our data suggest that mdfw and Cdh23(v) are allelic, (C) 2001 Academic Press.