Probing the non-mevalonate pathway to phytol in the cyanobacterium Synechocystis sp. UTEX 2470 using deuterium-labeled glucose

被引：11

作者：

Proteau, PJ ^{[1
]}

机构：

[1] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA

来源：

TETRAHEDRON LETTERS | 1998年 / 39卷 / 51期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0040-4039(98)02113-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The biosynthesis of phytol in the cyanobacterium Synechocystis sp. UTEX 2470 was examined using 1-H-2-D-glucose, 2-H-2-D-glucose, 2,3,4,6,6-H-2(5)-D-glucose, and 1,2,3,4,5,6,6-H-2(7)-D-glucose. Analysis of deuterium NMR spectra indicated that deuterium from the glucose precursors labels C1, C4, and C5 of the IPP units of phytol, while no labeling is observed at C2 of the IPP derived units. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：9373 / 9376

页数：4

共 10 条

[1] Incorporation of 2-C-methyl-D-erythritol, a putative isoprenoid precursor in the mevalonate-independent pathway, into ubiquinone and menaquinone of Escherichia coli [J].

Duvold, T ;

Cali, P ;

Bravo, JM ;

Rohmer, M .

TETRAHEDRON LETTERS, 1997, 38 (35) :6181-6184

[2]

Kaneko T, 1996, DNA Res, V3, P109

[3] Genetic and biochemical evidence for distinct key functions of two highly divergent GAPDH genes in catabolic and anabolic carbon flow of the cyanobacterium Synechocystis sp. PCC 6803 [J].

Koksharova, O ;

Schubert, M ;

Shestakov, S ;

Cerff, R .

PLANT MOLECULAR BIOLOGY, 1998, 36 (01) :183-194

[4] Direct formation of 2-C-methyl-D-erythritol 4-phosphate from 1-deoxy-D-xylulose 5-phosphate by 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a new enzyme in the non-mevalonate pathway to isopentenyl diphosphate [J].

Kuzuyama, T ;

Takahashi, S ;

Watanabe, H ;

Seto, H .

TETRAHEDRON LETTERS, 1998, 39 (25) :4509-4512

[5]

Lichtenthaler HK, 1998, FETT-LIPID, V100, P128

[6] Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants [J].

Lichtenthaler, HK ;

Rohmer, M ;

Schwender, J .

PHYSIOLOGIA PLANTARUM, 1997, 101 (03) :643-652

[7] Biosynthesis of phytol in the cyanobacterium Synechocystis sp. UTEX 2470:: Utilization of the non-mevalonate pathway [J].

Proteau, PJ .

JOURNAL OF NATURAL PRODUCTS, 1998, 61 (06) :841-843

[8] Glyceraldehyde 3-phosphate and pyruvate as precursors of isoprenic units in an alternative non-mevalonate pathway for terpenoid biosynthesis [J].

Rohmer, M ;

Seemann, M ;

Horbach, S ;

BringerMeyer, S ;

Sahm, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (11) :2564-2566

[9]

WALSH C, 1979, ENZY REACTIONS MECH

[10] ENZYME STEREOSPECIFICITIES FOR NICOTINAMIDE NUCLEOTIDES [J].

YOU, KS ;

ARNOLD, LJ ;

ALLISON, WS ;

KAPLAN, NO .

TRENDS IN BIOCHEMICAL SCIENCES, 1978, 3 (12) :265-268

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