Fluorometric determination of sulrite and nitrite in aqueous samples using a novel detection unit of a microfluidic device

被引：31

作者：

Fujii, S ^{[1
]}

Tokuyama, T ^{[1
]}

Abo, M ^{[1
]}

Okubo, A ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Tokyo 1138657, Japan

来源：

ANALYTICAL SCIENCES | 2004年 / 20卷 / 01期

关键词：

D O I：

10.2116/analsci.20.209

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On-chip fluorescence determination of sulfite and nitrite with N-(9-acridinyl)maleimide (NAM) and 2,3-diaminonaphthalene (DAN) has been developed using a novel fluorescence detection unit for microchip analysis. Usually, these fluorescence reagents are derivatized and detected separately in microchip analysis because different fluorescence wavelengths are emitted. The proposed fluorescence detection unit has optical fibers with no optical filter, and plural wavelengths of fluorescence were detected sensitively, even in the microchip. In this study, the simultaneous determination of sulfite and nitrite in environmental samples was performed with a polymer microchip analysis system. The calibration curves of sulfite and nitrite showed linear relations (R-2 = 0.998 (sulfite) and R-2 = 0.990 (nitrite)), and the relative standard deviations (RSD) for 4 runs were 2.1% (20 muM sulfite) and 1.3% (20 muM nitrite), respectively. The proposed method was applied to the recovery test of sulfite and nitrite in environmental samples.

引用

页码：209 / 212

页数：4

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