DNA Damage Caused by Ionizing Radiation in Embryonic Diploid Fibroblasts WI-38 Induces Both Apoptosis and Senescence

被引：28

作者：

Cmielova, J. ^{[1
]}

Havelek, R. ^{[1
]}

Jiroutova, A. ^{[1
]}

Kohlerova, R. ^{[1
]}

Seifrtova, M. ^{[1
]}

Muthna, D. ^{[1
]}

Vavrova, J. ^{[2
]}

Rezacova, M. ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Med, Dept Med Biochem, Simkova 870, Hradec Kralove 50038 1, Czech Republic

[2] Univ Def Brno, Dept Radiol, Fac Mil Hlth Sci Hradec Kralove, Brno, Czech Republic

来源：

PHYSIOLOGICAL RESEARCH | 2011年 / 60卷 / 04期

关键词：

DNA damage; Ionizing radiation; Senescence; Stem cells; STEM-CELLS; PREMATURE SENESCENCE; CELLULAR SENESCENCE; ACTIVATION; CYCLE; PHOSPHORYLATION; MECHANISMS; P53; ATM;

D O I：

10.33549/physiolres.932083

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Cellular response to ionizing radiation-induced damage depends on the cell type and the ability to repair DNA damage. Some types of cells undergo apoptosis, whereas others induce a permanent cell cycle arrest and do not proliferate. Our study demonstrates two types of response of embryonic diploid fibroblasts WI-38 to ionizing radiation. In the WI-38 cells p53 is activated, protein p21 increases, but the cells are arrested in G2 phase of cell cycle. Some of the cells die by apoptosis, but in remaining viable cells p16 increases, senescence associated DNA damage foci occur, and senescence-associated beta-galactosidase activity increases, which indicate stress-induced premature senescence.

引用

页码：667 / 677

页数：11

共 25 条

[1] DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation [J].