Functional characterization of SOR1, a gene required for resistance to photosensitizing toxins in the fungus Cercospora nicotianae

被引：42

作者：

Ehrenshaft, M ^{[1
]}

Chung, KR ^{[1
]}

Jenns, AE ^{[1
]}

Daub, ME ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA

来源：

CURRENT GENETICS | 1999年 / 34卷 / 06期

基金：

美国国家科学基金会;

关键词：

photosensitizer resistance; singlet oxygen resistance; Cercospora spp; cercosporin;

D O I：

10.1007/s002940050423

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Cercospora nicotianae SORI gene is required for resistance to singlet oxygen-generating photosensitizers. SORI was characterized in the wild-type and in five photosensitizer-sensitive mutant strains which are complemented to photosensitizer resistance by transformation with SORI. Sequence analysis determined that three of the mutants contain SORI copies with mutations encoding substitutions in the protein-coding sequence; however, two other mutants had wild-type SOR1 protein and promoter sequences. All five mutants accumulate SORI mRNA at levels comparable to that of the wild-type strain. In the wild-type strain, SORI accumulation is enhanced two-fold by light, but is unaffected by the presence of cercosporin, the photosensitizer synthesized by C. nicotianae. Southern analysis indicates that SORI is present in other fungi that synthesize structurally related perylenequinone photosensitizers.

引用

页码：478 / 485

页数：8

共 22 条

[1] A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family [J].

Braun, EL ;

Fuge, EK ;

Padilla, PA ;

WernerWashburne, M .

JOURNAL OF BACTERIOLOGY, 1996, 178 (23) :6865-6872

[2]

Chung KR, 1996, GENETICS, V142, P259

[3]

DAUB ME, 1982, PHYTOPATHOLOGY, V72, P370, DOI 10.1094/Phyto-72-370

[4] LIGHT-INDUCED PRODUCTION OF SINGLET OXYGEN AND SUPEROXIDE BY THE FUNGAL TOXIN, CERCOSPORIN [J].

DAUB, ME ;

HANGARTER, RP .

PLANT PHYSIOLOGY, 1983, 73 (03) :855-857

[5]

DAUB ME, 1993, PHYSIOL PLANTARUM, V89, P227, DOI 10.1111/j.1399-3054.1993.tb01810.x

[6] REDUCTIVE DETOXIFICATION AS A MECHANISM OF FUNGAL RESISTANCE TO SINGLET OXYGEN-GENERATING PHOTOSENSITIZERS [J].

DAUB, ME ;

LEISMAN, GB ;

CLARK, RA ;

BOWDEN, EF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) :9588-9592

[7] PEROXIDATION OF TOBACCO MEMBRANE-LIPIDS BY THE PHOTOSENSITIZING TOXIN, CERCOSPORIN [J].

DAUB, ME .

PLANT PHYSIOLOGY, 1982, 69 (06) :1361-1364

[8] CHANGES IN TOBACCO CELL-MEMBRANE COMPOSITION AND STRUCTURE CAUSED BY CERCOSPORIN [J].

DAUB, ME ;

BRIGGS, SP .

PLANT PHYSIOLOGY, 1983, 71 (04) :763-766

[9]

Daub ME, 1995, ACS SYM SER, V616, P201

[10] SOR1, a gene required for photosensitizer and singlet oxygen resistance in Cercospora fungi, is highly conserved in divergent organisms [J].

Ehrenshaft, M ;

Jenns, AE ;

Chung, KR ;

Daub, ME .

MOLECULAR CELL, 1998, 1 (04) :603-609

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