Isolation and characterization of a cDNA clone from Arabidopsis thaliana with partial sequence similarity to integrins

被引：37

作者：

Nagpal, P ^{[1
]}

Quatrano, RS ^{[1
]}

机构：

[1] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

来源：

GENE | 1999年 / 230卷 / 01期

基金：

美国国家科学基金会;

关键词：

integrin; plant; plasma membrane; transmembrane;

D O I：

10.1016/S0378-1119(99)00068-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An Arabidopsis thaliana cDNA, called At14a, was isolated by immunoscreening an expression library with an anti-integrin antibody. The At14a cDNA is 1459 nucleotides and has an open reading frame encoding a protein of 385 amino acids and a predicted molecular weight of 43 kDa. At14a has a small domain that has sequence similarities to integrins from fungi, insects and humans. Transcripts of At14a are found in all Arabidopsis tissues examined, and when expressed as an epitope tagged fusion protein in transgenic plants, At14a localizes partly to the plasma membrane. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：33 / 40

页数：8

相关论文

共 31 条

[1] AMINO-ACID-SEQUENCE OF THE HUMAN FIBRONECTIN RECEPTOR [J].

ARGRAVES, WS ;

SUZUKI, S ;

ARAI, H ;

THOMPSON, K ;

PIERSCHBACHER, MD ;

RUOSLAHTI, E .

JOURNAL OF CELL BIOLOGY, 1987, 105 (03) :1183-1190

[2]

BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194

[3] THE DROSOPHILA PS2 ANTIGEN IS AN INVERTEBRATE INTEGRIN THAT, LIKE THE FIBRONECTIN RECEPTOR, BECOMES LOCALIZED TO MUSCLE ATTACHMENTS [J].

BOGAERT, T ;

BROWN, N ;

WILCOX, M .

CELL, 1987, 51 (06) :929-940

[4] Molecular evolution of integrins: Genes encoding integrin beta subunits from a coral and a sponge [J].

Brower, DL ;

Brower, SM ;

Hayward, DC ;

Ball, EE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (17) :9182-9187

[5] High affinity RGD-binding sites at the plasma membrane of Arabidopsis thaliana links the cell wall [J].

Canut, H ;

Carrasco, A ;

Galaud, JP ;

Cassan, C ;

Bouyssou, H ;

Vita, N ;

Ferrara, P ;

Pont-Lezica, R .

PLANT JOURNAL, 1998, 16 (01) :63-71

[6]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[7] ARABIDOPSIS THALIANA MUTANT THAT DEVELOPS AS A LIGHT-GROWN PLANT IN THE ABSENCE OF LIGHT [J].

CHORY, J ;

PETO, C ;

FEINBAUM, R ;

PRATT, L ;

AUSUBEL, F .

CELL, 1989, 58 (05) :991-999

[8] INTEGRINS AND SIGNAL-TRANSDUCTION PATHWAYS - THE ROAD TAKEN [J].

CLARK, EA ;

BRUGGE, JS .

SCIENCE, 1995, 268 (5208) :233-239

[9] THE PLASMA-MEMBRANE OF ARABIDOPSIS-THALIANA CONTAINS A MERCURY-INSENSITIVE AQUAPORIN THAT IS A HOMOLOG OF THE TONOPLAST WATER CHANNEL PROTEIN TIP [J].

DANIELS, MJ ;

MIRKOV, TE ;

CHRISPEELS, MJ .

PLANT PHYSIOLOGY, 1994, 106 (04) :1325-1333

[10]

Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670

← 1 2 3 4 →