Isolation and characterization of a cDNA clone from Arabidopsis thaliana with partial sequence similarity to integrins

被引:37
作者
Nagpal, P [1 ]
Quatrano, RS [1 ]
机构
[1] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
基金
美国国家科学基金会;
关键词
integrin; plant; plasma membrane; transmembrane;
D O I
10.1016/S0378-1119(99)00068-2
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
An Arabidopsis thaliana cDNA, called At14a, was isolated by immunoscreening an expression library with an anti-integrin antibody. The At14a cDNA is 1459 nucleotides and has an open reading frame encoding a protein of 385 amino acids and a predicted molecular weight of 43 kDa. At14a has a small domain that has sequence similarities to integrins from fungi, insects and humans. Transcripts of At14a are found in all Arabidopsis tissues examined, and when expressed as an epitope tagged fusion protein in transgenic plants, At14a localizes partly to the plasma membrane. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:33 / 40
页数:8
相关论文
共 31 条
[31]  
ZHU JK, 1993, PLANT J, V3, P637, DOI 10.1111/j.1365-313X.1993.00637.x