共 21 条
The ras-related protein Ral is monoglucosylated by Clostridium sordellii lethal toxin
被引:46
作者:
Hofmann, F
[1
]
Rex, G
[1
]
Aktories, K
[1
]
Just, I
[1
]
机构:
[1] UNIV FREIBURG,INST PHARMAKOL & TOXIKOL,D-79104 FREIBURG,GERMANY
关键词:
D O I:
10.1006/bbrc.1996.1470
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Clostridium sordellii lethal toxin (LT), a cytotoxin which causes preferential destruction of the actin cytoskeleton, has been recently identified as glucosyltransferase to modify the low molecular mass GTPases Rac, Ras and Rap. We report here on LT produced by C. sordellii strain 6018 which glucosylates in addition lo Rac, Ras and Rap the Ral protein. LT from strain VPI9048 however does not glucosylate Ral. Besides recombinant Ral, cellular Ral is also substrate. In the GDP-bound form, Ral is a superior substrate to the GTP form. Acceptor amino acid for glucose is threonine-46 which is equivalent to threonine-35 in H-Ras located in the effector region. The Ral-glucosylating toxin is a novel isoform of Ras-modifying clostridial cytotoxins. (C) 1996 Academic Press, Inc.
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页码:77 / 81
页数:5
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