A novel fluorescent aptasensor for selective and sensitive detection of digoxin based on silica nanoparticles

被引：37

作者：

Emrani, Ahmad Sarreshtehdar ^{[1
]}

Taghdisi, Seyed Mohammad ^{[2
]}

Danesh, Noor Mohammad ^{[3
,4
]}

Jalalian, Seyed Hamid ^{[4
,5
]}

Ramezani, Mohammad ^{[4
]}

Abnous, Khalil ^{[6
]}

机构：

[1] Mashhad Univ Med Sci, Ghaem Hosp, Cardiovasc Res Ctr, Mashhad, Iran

[2] Mashhad Univ Med Sci, Sch Pharm, Targeted Drug Delivery Res Ctr, Mashhad, Iran

[3] Res Inst Sci & New Technol, Mashhad, Iran

[4] Mashhad Univ Med Sci, Sch Pharm, Nanotechnol Res Ctr, Mashhad, Iran

[5] Acad Ctr Educ Culture & Res, Mashhad Branch, Mashhad, Iran

[6] Mashhad Univ Med Sci, Sch Pharm, Pharmaceut Res Ctr, Mashhad, Iran

来源：

ANALYTICAL METHODS | 2015年 / 7卷 / 09期

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; GOLD NANOPARTICLE; ULTRASENSITIVE DETECTION; COLORIMETRIC APTASENSOR; ENHANCED FLUORESCENCE; APTAMER BIOSENSOR; MASS-SPECTROMETRY; SURFACE; SAMPLES; ASSAY;

D O I：

10.1039/c5ay00622h

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Digoxin is a useful drug in the treatment of heart failure. The therapeutic index of digoxin is very narrow. Thus, its detection in serum is of remarkable interest. In this study we designed a sensitive and selective fluorescent aptasensor for detection of digoxin based on silica nanoparticles (SNPs) coated with streptavidin. In the absence of digoxin, the fluorescence of the SNP-double stranded DNA (dsDNA) complex is strong. Upon addition of digoxin, the aptamer binds to its target, leading to release of the labeled-complementary strand from dsDNA and SNPs and decrease of the fluorescence intensity. This aptasensor showed a high selectivity toward digoxin and a limit of detection as low as 566 pM was obtained. This sensing platform is expected to be used successfully for detection of other drugs and biomolecules.

引用

页码：3814 / 3818

页数：5

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