CYP450 biosensors based on gold chips for antiepileptic drugs determination

被引：22

作者：

Alonso-Lomillo, M. A. ^{[1
]}

Gonzalo-Ruiz, J. ^{[2
]}

Dominguez-Renedo, O. ^{[1
]}

Munoz, F. J. ^{[2
]}

Arcos-Martinez, M. J. ^{[1
]}

机构：

[1] Univ Burgos, Fac Sci, Dept Analyt Chem, Burgos, Spain

[2] IMB CSIC, Microelect Inst Barcelona, Bellaterra, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 11期

关键词：

biosensor; microelectronic techniques; screen-printed Ag/AgCl reference electrode; phenobarbital; cytochrome p450 2B4;

D O I：

10.1016/j.bios.2008.01.030

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Three-electrode configuration chips containing a Pt, An and a screen-printed Ag/AgCl as counter, working and reference electrode, respectively, have been developed. Selective determination of Phenobarbital (PB) has been carried out by Cytochrome P450 2134 (CYP450) immobilization into a polypyrrole matrix onto the gold working electrode. Chronoamperometric experiments show a PB diffusion coefficient of 2.42 x 10(-6) cm(2) s(-1), a reproducibility and repeatability in terms of residual standard deviation (RSD) of 13% and 5.51%, respectively, and a limit of detection (LOD) of 0.289 mu mol dm(-3) (alpha = beta = 0.05) for the developed CYP450-biosensor chip. Its performance has been showed by the determination of PB in pharmaceutical drugs. HPLC has been used as reference technique. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：1733 / 1737

页数：5

共 32 条

[1]

ALONSOLOMILLO A, 2005, ANAL CHIM ACTA, V547, P209

[2] Cytochrome P450 biosensors - a review [J].

Bistolas, N ;

Wollenberger, U ;

Jung, C ;

Scheller, FW .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (12) :2408-2423

[3]

BLANCO M, 1994, AVANCES QUIMIOINETRI

[4] Simultaneous spectrophotometric determination of phenobarbital, phenytoin and methylphenobarbital in pharmaceutical preparations by using partial least-squares and principal component regression multivariate calibration [J].

Boeris, MS ;

Luco, JM ;

Olsina, RA .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2000, 24 (02) :259-271

[5] Simultaneous detection of three drugs labeled by cationic metal complexes at a nafion-loaded carbon paste electrode [J].

Bordes, AL ;

Schöllhorn, B ;

Limoges, B ;

Degrand, C .

TALANTA, 1999, 48 (01) :201-208

[6] Simultaneous determination of anticonvulsants and their principal metabolites by HPLC [J].

Cavazos, MDLLS ;

de la Cruz, VT ;

de Torres, NW .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2005, 28 (05) :693-704

[7]

Cosnier S, 1998, ELECTROANAL, V10, P808, DOI 10.1002/(SICI)1521-4109(199809)10:12<808::AID-ELAN808>3.3.CO

[8]

2-B

[9] Evaluation of miniaturised solid state reference electrodes on a silicon based component [J].

Desmond, D ;

Lane, B ;

Alderman, J ;

Glennon, JD ;

Diamond, D ;

Arrigan, DWM .

SENSORS AND ACTUATORS B-CHEMICAL, 1997, 44 (1-3) :389-396

[10] A bioelectrochemical polypyrrole-containing Fe(CN)63- interface for the design of a NAD-dependent reagentless biosensor [J].

Gros, P ;

Comtat, M .

BIOSENSORS & BIOELECTRONICS, 2004, 20 (02) :204-210

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