Differential subcellular localization of hZip1 in adherent and non-adherent cells

被引：53

作者：

Milon, B

Dhermy, D

Pountney, D

Bourgeois, M

Beaumont, C

机构：

[1] Fac Xavier Bichat, INSERM U409, F-75018 Paris 18, France

[2] Fac Xavier Bichat, IFR Claude Bernard, F-75018 Paris 18, France

来源：

FEBS LETTERS | 2001年 / 507卷 / 03期

关键词：

zinc; hZip1; ZIP family; endoplasmic reticulum; plasma membrane;

D O I：

10.1016/S0014-5793(01)02950-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two human divalent cation transporters of the ZIP family, hZip1 and hZip2, homologous to Irt1 (Arabidopsis thaliana), the first identified member, have been described. They were shown by transfection into K562 cells to be localized at the plasma membrane and to mediate zinc uptake. Here we report a differential subcellular localization of hZip1 according to cell type. By transient expressions of EGFP-hZip1, FLAG-tagged or native hZip1, we observed that hZip1 has a vesicular localization in COS-7 cells or in several epithelial cell lines, corresponding partially to the endoplasmic reticulum. Using anti-hZip1 antibodies, we confirmed the intracellular localization of the endogenous protein in PC-3, a prostate cancer cell line. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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页码：241 / 246

页数：6

相关论文

共 13 条

[1] Elimination of zinc from synaptic vesicles in the intact mouse brain by disruption of the ZnT3 gene [J].

Cole, TB ;

Wenzel, HJ ;

Kafer, KE ;

Schwartzkroin, PA ;

Palmiter, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (04) :1716-1721

[2] Evidence for a zinc uptake transporter in human prostate cancer cells which is regulated by prolactin and testosterone [J].

Costello, LC ;

Liu, YY ;

Zou, J ;

Franklin, RB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (25) :17499-17504

[3] Integrative aspects of zinc transporters [J].

Cousins, RJ ;

McMahon, RJ .

JOURNAL OF NUTRITION, 2000, 130 (05) :1384S-1387S

[4] Functional expression of the human hZIP2 zinc transporter [J].

Gaither, LA ;

Eide, DJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (08) :5560-5564

[5] The human ZIP1 transporter mediates zinc uptake in human K562 erythroleukemia cells [J].

Gaither, LA ;

Eide, DJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (25) :22258-22264

[6] The ZIP family of metal transporters [J].

Guerinot, ML .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1465 (1-2) :190-198

[7] GOLGI-APPARATUS IN CHICK SKELETAL-MUSCLE - CHANGES IN ITS DISTRIBUTION DURING END PLATE DEVELOPMENT AND AFTER DENERVATION [J].

JASMIN, BJ ;

CARTAUD, J ;

BORNENS, M ;

CHANGEUX, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) :7218-7222

[8] Differential expression of chicken dimerization cofactor of hepatocyte nuclear factor-1 (DcoH) and its novel counterpart, DcoHα [J].

Kim, H ;

You, S ;

Foster, LK ;

Farris, J ;

Choi, YJ ;

Foster, DN .

BIOCHEMICAL JOURNAL, 2001, 354 (03) :645-653

[9] The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range [J].

Korshunova, YO ;

Eide, D ;

Clark, WG ;

Guerinot, ML ;

Pakrasi, HB .

PLANT MOLECULAR BIOLOGY, 1999, 40 (01) :37-44

[10] Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification [J].

Kurtzman, AL ;

Schechter, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (10) :5602-5607