Rab3a controls exocytosis in cholecystokinin-secreting cells

被引：12

作者：

Gevrey, JC

Laurent, S

Saurin, JC

Némoz-Gaillard, E

Regazzi, R

Chevrier, AM

Chayvialle, JA

Abello, J ^{[1
]}

机构：

[1] Hop Edouard Herriot, INSERM, Unite 45, F-69437 Lyon 3, France

[2] Inst Biol Cellulaire & Morphol, CH-1005 Lausanne, Switzerland

来源：

FEBS LETTERS | 2001年 / 503卷 / 01期

关键词：

exocytosis; rab3; cholecystokinin; enteroendocrine cell;

D O I：

10.1016/S0014-5793(01)02683-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of rab3A and rab3D isoforms in the enteroendocrine, cholecystokinin-secreting, cell lines STC-1 and GLUTag is here demonstrated. In contrast, rab3B is undetectable in these two cell lines, and rab3C is only slightly expressed in GLUTag cells. Using a transient co-transfection system with human growth hormone as reporter protein, we show that overexpression of the GTPase-deficient mutant rab3AQ81L, but not rab3DQ81L, significantly decreases human growth hormone secretory responses to various agonists in STC-1 cells. These results indicate that endocrine cell lines of intestinal origin express rab3A and rab3D proteins, but the GTP-bound form of rab3A Only acts as a negative modulator in the control of cholecystokinin secretion from STC-1 cells. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：19 / 24

页数：6

共 41 条

[21] Expression of SNARE proteins in enteroendocrine cell lines and functional role of tetanus toxin-sensitive proteins in cholecystokinin release [J].