Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins

被引：3

作者：

Aokage, T ^{[1
]}

Ohsawa, I ^{[1
]}

Ohta, S ^{[1
]}

机构：

[1] Nippon Med Coll, Grad Sch Med, Inst Dev & Aging Sci, Dept Biochem & Cell Biol,Nakahara Ku, Kawasaki, Kanagawa 2118533, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 314卷 / 03期

关键词：

D O I：

10.1016/j.bbrc.2003.12.152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-x(L), and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). GFP but not EGFP seemed to promote cell death induced by pro-apoptotic Bax. Thus, GFP specifically promotes the aggregation of mitochondria when co-expressed with a member of the Bcl-2 family in association with apoptosis. (C) 2003 Elsevier Inc. All rights reserved.

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页码：711 / 716

页数：6

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