Enhanced fluorescence cyanide detection at physiologically lethal levels: Reduced ICT-based signal transduction

被引:370
作者
Badugu, R
Lakowicz, JR
Geddes, CD [1 ]
机构
[1] Univ Maryland, Sch Med, Ctr Fluorescence Spect, Dept Biochem & Mol Biol,Med Biotechnol Ctr, 725 W Lombard St, Baltimore, MD 21201 USA
[2] Univ Maryland, Inst Biotechnol, Inst Fluorescence, Lab Adv Fluorescence Spect,Med Biotechnol Ctr, Baltimore, MD 21201 USA
关键词
D O I
10.1021/ja044421i
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Three water-soluble fluorescent probes have been specifically designed to determine free cyanide concentrations up to physiologically lethal levels, > 20 mu M. The probes have been designed in such a way as to afford many notable sensing features, which render them unique with regard to signal transduction, photophysical characteristics, and their application to physiological cyanide determination and safeguard. The probes are readily able to reversibly bind free aqueous cyanide with dissociation constants around 4 mu M-3. Subsequent cyanide binding modulates the intramolecular charge transfer within the probes, a change in the electronic properties within the probes, resulting in enhanced fluorescence optical signals as a function of increased solution cyanide concentration. The ground-state chelation with cyanide produces wavelength shifts, which also enable the probes to sense cyanide in both an excitation and emission ratiometric manner, in addition to enhanced fluorescence signaling. This has enabled a generic cyanide sensing platform to be realized that is not dependent on fluorescent probe concentration, probe photodegradation, or fluctuations in the intensity of any employed excitation sources, ideal for remote cyanide sensing applications. Further, the > 600 nm fluorescence emission of the probes potentially allows for enhanced fluorescence ratiometric cyanide sensing in the optical window of tissues and blood, facilitating their use for the transdermal monitoring of cyanide for mammalian safeguard or postmortem in fire victims, both areas of active research.
引用
收藏
页码:3635 / 3641
页数:7
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