Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes

被引:33
作者
Jakeman, DL [1 ]
Mitchell, DJ [1 ]
Shuttleworth, WA [1 ]
Evans, JNS [1 ]
机构
[1] Washington State Univ, Dept Biochem & Biophys, Pullman, WA 99163 USA
关键词
EPSP synthase; fast freezing; narrowing linewidths; P-31; MMR; solid-state NMR; trehalose;
D O I
10.1023/A:1008305118426
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of P-31 resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 +/- 12 Hz and 125 +/- 7 Hz respectively. by a variety of methods involving additives and freezing techniques.
引用
收藏
页码:417 / 421
页数:5
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