Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes

被引：33

作者：

Jakeman, DL ^{[1
]}

Mitchell, DJ ^{[1
]}

Shuttleworth, WA ^{[1
]}

Evans, JNS ^{[1
]}

机构：

[1] Washington State Univ, Dept Biochem & Biophys, Pullman, WA 99163 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 1998年 / 12卷 / 03期

关键词：

EPSP synthase; fast freezing; narrowing linewidths; P-31; MMR; solid-state NMR; trehalose;

D O I：

10.1023/A:1008305118426

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of P-31 resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 +/- 12 Hz and 125 +/- 7 Hz respectively. by a variety of methods involving additives and freezing techniques.

引用

页码：417 / 421

页数：5

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