Bright and stable near-infrared fluorescent protein for in vivo imaging

被引：547

作者：

Filonov, Grigory S. ^{[1
,2
]}

Piatkevich, Kiryl D. ^{[1
,2
]}

Ting, Li-Min ^{[3
,4
]}

Zhang, Jinghang ^{[5
]}

Kim, Kami ^{[3
,4
]}

Verkhusha, Vladislav V. ^{[1
,2
]}

机构：

[1] Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10467 USA

[2] Albert Einstein Coll Med, Gruss Lipper Biophoton Ctr, Bronx, NY 10467 USA

[3] Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA

[4] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA

[5] Albert Einstein Coll Med, Flow Cytometry Core Facil, Bronx, NY 10467 USA

来源：

NATURE BIOTECHNOLOGY | 2011年 / 29卷 / 08期

基金：

美国国家卫生研究院;

关键词：

ELECTROSTATIC INTERACTIONS; PHYTOCHROME; BACTERIOPHYTOCHROME; STABILITY; EXPRESSION; BACTERIA; CELLS; CHAIN;

D O I：

10.1038/nbt.1918

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Imaging biological processes in mammalian tissues will be facilitated by fluorescent probes with excitation and emission bands within the near-infrared optical window of high transparency(1). Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of the chromophore biliverdin and has higher effective brightness, intracellular stability and photostability than earlier phytochrome-derived fluorescent probes. Compared with far-red GFP-like proteins, iRFP has a substantially higher signal-to-background ratio in a mouse model due to its infrared-shifted spectra.

引用

页码：757 / U133

页数：7

共 34 条

[11] Biliverdin binds covalently to Agrobacterium phytochrome Agp1 via its ring a vinyl side chain [J].

Lamparter, T ;

Michael, N ;

Caspani, O ;

Miyata, T ;

Shirai, K ;

Inomata, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (36) :33786-33792

[12] CONTINUOUS FLUORESCENCE ASSAY OF PHYTOCHROME ASSEMBLY IN-VITRO [J].

LI, LM ;

MURPHY, JT ;

LAGARIAS, JC .

BIOCHEMISTRY, 1995, 34 (24) :7923-7930

[13] Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals [J].

Lin, Michael Z. ;

McKeown, Michael R. ;

Ng, Ho-Leung ;

Aguilera, Todd A. ;

Shaner, Nathan C. ;

Campbell, Robert E. ;

Adams, Stephen R. ;

Gross, Larry A. ;

Ma, Wendy ;

Alber, Tom ;

Tsien, Roger Y. .

CHEMISTRY & BIOLOGY, 2009, 16 (11) :1169-1179

[14] A protocol for rapid generation of recombinant adenoviruses using the AdEasy system [J].

Luo, Jinyong ;

Deng, Zhong-Liang ;

Luo, Xiaoji ;

Tang, Ni ;

Song, Wen-Xin ;

Chen, Jin ;

Sharff, Katie A. ;

Luu, Hue H. ;

Haydon, Rex C. ;

Kinzler, Kenneth W. ;

Vogelstein, Bert ;

He, Tong-Chuan .

NATURE PROTOCOLS, 2007, 2 (05) :1236-1247

[15] PROTEIN DENATURATION WITH GUANIDINE-HYDROCHLORIDE OR UREA PROVIDES A DIFFERENT ESTIMATE OF STABILITY DEPENDING ON THE CONTRIBUTIONS OF ELECTROSTATIC INTERACTIONS [J].

MONERA, OD ;

KAY, CM ;

HODGES, RS .

PROTEIN SCIENCE, 1994, 3 (11) :1984-1991

[16] Far-Red Fluorescent Protein Excitable with Red Lasers for Flow Cytometry and Superresolution STED Nanoscopy [J].

Morozova, Kateryna S. ;

Piatkevich, Kiryl D. ;

Gould, Travis J. ;

Zhang, Jinghang ;

Bewersdorf, Joerg ;

Verkhusha, Vladislav V. .

BIOPHYSICAL JOURNAL, 2010, 99 (02) :L13-L15

[17] Evolutionary Gain of Function for the ER Membrane Protein Sec62 from Yeast to Humans [J].

Mueller, Linda ;

de Escauriaza, Maria Diaz ;

Lajoie, Patrick ;

Theis, Melanie ;

Jung, Martin ;

Mueller, Anika ;

Burgard, Carsten ;

Greiner, Markus ;

Snapp, Erik L. ;

Dudek, Johanna ;

Zimmermann, Richard .

MOLECULAR BIOLOGY OF THE CELL, 2010, 21 (05) :691-703

[18]

Ntziachristos V, 2010, NAT METHODS, V7, P603, DOI [10.1038/NMETH.1483, 10.1038/nmeth.1483]

[19] A Brief History of Phytochromes [J].

Rockwell, Nathan C. ;

Lagarias, J. Clark .

CHEMPHYSCHEM, 2010, 11 (06) :1172-1180

[20] SURFACE ELECTROSTATIC INTERACTIONS CONTRIBUTE LITTLE TO STABILITY OF BARNASE [J].

SALI, D ;

BYCROFT, M ;

FERSHT, AR .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 220 (03) :779-788

← 1 2 3 4 →