Characterization of a lympho-inhibitory peptide produced by Mycoplasma bovis

被引：38

作者：

Vanden Bush, TJ ^{[1
]}

Rosenbusch, RF ^{[1
]}

机构：

[1] Iowa State Univ Sci & Technol, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 315卷 / 02期

关键词：

Mycoplasma bovis; lymphocyte; immunosuppression; peptide; PBMC;

D O I：

10.1016/j.bbrc.2004.01.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mycoplasma bovis is able to inhibit the mitogen-induced proliferation of bovine lymphocytes. Herein is described the isolation of an immuno-inhibitory peptide from M. bovis. Using size exclusion chromatography, three lympho-suppressive fractions were isolated from M. bovis free supernatant. MALDI-TOF analysis revealed a common peak throughout the suppressive fractions. The purest of these fractions was subjected to N-terminal sequencing; revealing an 84% homologous match with the C-terminus of the M. bovis surface protein VspL (variable surface protein-L). A recombinant of the 26 amino acid peptide was also able to suppress Concanavalin A (ConA)-induced proliferation of bovine lymphocytes. This describes a unique immunosuppressive peptide produced by the bovine respiratory pathogen, M. bovis. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：336 / 341

页数：6