Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells

被引：21

作者：

Zandi, Keivan ^{[1
]}

Lani, Rafidah ^{[1
]}

Wong, Pooi-Fong ^{[2
]}

Teoh, Boon-Teong ^{[1
]}

Sam, Sing-Sin ^{[1
]}

Johari, Jefree ^{[1
]}

Mustafa, Mohd Rais ^{[2
]}

AbuBakar, Sazaly ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Med Microbiol, TIDREC, Kuala Lumpur 50603, Malaysia

[2] Univ Malaya, Fac Med, Dept Pharmacol, Kuala Lumpur 50603, Malaysia

来源：

MOLECULES | 2012年 / 17卷 / 03期

关键词：

antiviral; dengue; flavones; flavonoid; molecule; ANTIVIRAL ACTIVITY; IN-VITRO;

D O I：

10.3390/molecules17032437

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 mu g/mL) significantly increased the number of DENV-2 foci by 35.66% +/- 1.52 and 49.66% +/- 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33% +/- 2.11 and 89% +/- 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 mu g/mL) did not promote or inhibit Vero cell proliferation. The CC50 value of flavone against Vero cells was 446 mu g/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.

引用

页码：2437 / 2445

页数：9

共 20 条

[1] Dengue virus type 2 envelope protein displayed as recombinant phage attachment protein reveals potential cell binding sites [J].